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AAV-CMVc-Cas9

Plasmid

#106431

Purpose

Expresses SpCas9 from the CMVc promoter in AAV backbone

Depositor

Juan Belmonte

Publication

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Cas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMVc

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

Plasmid

#133027

Purpose

Expresses EGFP-tagged late endosome marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB7A (RAB7A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV/myc/Nuc-hOGG1

Plasmid

#18709

Depositor

David Sidransky

Publication

Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

Insert

8-oxoguanine DNA glycosylase (OGG1 Human)

Expression

Mammalian

Mutation

N/A

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Mutation

R175H

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS2-UTX-F-MT2

Plasmid

#40619

Purpose

Expression of enzyme-dead UTX in mammalian cells

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Mutation

changed Histidine 1146 and Glutamic Acid 1148 to …

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
AAV-CMVc-Cas9

Plasmid

#106431

Purpose

Expresses SpCas9 from the CMVc promoter in AAV backbone

Depositor

Juan Belmonte

Publication

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

Cas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMVc

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CoV2-Spike-D614G

Plasmid

#177960

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )

Expression

Mammalian

Mutation

D614G

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

Plasmid

#167582

Purpose

Express the ATP sensor GRAB_ATP1.0 in mammalian cells

Depositor

Yulong Li

Publication

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDONR223-PRKAA1

Plasmid

#23871

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAA1 (PRKAA1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pET StrepII-StrepII TEV LIC cloning vector (2RRT)

Plasmid

#55215

Depositor

Scott Gradia

Type

Empty backbone

Tags

2xStrep II and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Mutation

R175H

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS2-UTX-F-MT2

Plasmid

#40619

Purpose

Expression of enzyme-dead UTX in mammalian cells

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Mutation

changed Histidine 1146 and Glutamic Acid 1148 to …

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
CoV2-Spike-D614G

Plasmid

#177960

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )

Expression

Mammalian

Mutation

D614G

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

Plasmid

#167582

Purpose

Express the ATP sensor GRAB_ATP1.0 in mammalian cells

Depositor

Yulong Li

Publication

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pWZL Neo Myr Flag AXL

Plasmid

#20428

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AXL (AXL Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDONR223-FLT4

Plasmid

#23923

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT4 (FLT4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKAA1

Plasmid

#23871

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAA1 (PRKAA1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCMV-Mm.cargo(Cas9)

Plasmid

#174865

Purpose

Expresses mouse Peg10 cargoRNA encoding SpCas9

Depositor

Feng Zhang

Publication

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

SpCas9 flanked by MmPeg10 UTRs

Tags

HA-SV40 NLS and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 V5 hHR23B

Plasmid

#13054

Depositor

Steven Grossman

Publication

Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Insert

hHR23B (RAD23B Human)

Tags

His and V5

Expression

Mammalian

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTR-PIP-FUCCI

Plasmid

#118621

Purpose

Gateway cloning vector for the cell cycle indicator PIP-FUCCI

Depositor

Jean Cook

Publication

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Gateway Cloning

Tags

mCherry and mVenus

Available since

April 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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