We narrowed to 5,844 results for: pCas

1. pH-Ubi-miniSdd7-nCas9-PBE

   Plasmid

   #204856

   Purpose

   For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd7-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   ZmUbi-1, OsU3, 35S

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCK340

   Plasmid

   #192639

   Purpose

   Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dSpG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   SoxS has R93A and S101A mutations and dSpG has se…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

   Plasmid

   #139086

   Purpose

   Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMIA3-Olônne

   Plasmid

   #214447

   Purpose

   Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   eSpCas9-P2A-mRuby2-P2A-tp53dn
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pL-CRISPR.SFFV.PAC

   Plasmid

   #57829

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-EF1a-SPdCas9-EGFP-2A-Blast

   Plasmid

   #71215

   Purpose

   dCas9 fused to EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-EGFP
   

   Use

   Lentiviral

   Tags

   dSpCas9-EGFP

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BPK2797

   Plasmid

   #72251

   Purpose

   Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, and T1337R

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330-sgRNA_Ago2_1

   Plasmid

   #73529

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pX330-sgRNA_Ago2_2

   Plasmid

   #73530

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pX330-NQL005-SOX2-sgRNA

    Plasmid

    #175553

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCCL-PGK-SPdCas9-BFP-EGFP

    Plasmid

    #66821

    Purpose

    dCas9 fused to BFP and EGFP

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-EGFP
    

    Tags

    dSpCas9-BFP-EGFP

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCK669

    Plasmid

    #192638

    Purpose

    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dxCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dxCas9-NG h…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330-NQL007-NANOG-sgRNA

    Plasmid

    #175555

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pU6-(BsaI)_CBh-Cas9

    Plasmid

    #135010

    Purpose

    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9

    Depositor

    Yosef Shaul

    Article

    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

    Insert

    humanized S. pyogenes Cas9
    

    Tags

    3xFLAG (N terminal on insert)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p-miR-Report + WT miR-181 BS in NLK 3UTR

    Plasmid

    #22790

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Wild-type miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)
    

    Use

    Luciferase

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pUDR211

    Plasmid

    #113870

    Purpose

    expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA3-HXT8 / sgRNA4-HXT14
    

    Expression

    Yeast

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUDR418

    Plasmid

    #113875

    Purpose

    expression of double Cas9 programming sgRNA11 targetting STL1

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    doble sgRNA11-STL1
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV10.F6.loxP

    Plasmid

    #63201

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p-miR-Report + MT miR-181 BS in NLK 3UTR

    Plasmid

    #22791

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Mutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)
    

    Use

    Luciferase

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pFB-Flag-MyoD(QQQ)-E12

    Plasmid

    #26003

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Mutation

    Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

    Available Since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge