We narrowed to 10,076 results for: pCas

1. pMMACE DEST CMV SpCas9

   Plasmid

   #206268

   Purpose

   DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9
   

   Use

   CRISPR; Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCaSper4 cyto roGFP2-Orp1

   Plasmid

   #64998

   Purpose

   Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAMBIA-FLS2prom::FLS2-3xmyc

   Plasmid

   #86158

   Purpose

   FLS2 expression

   Depositor

   Silke Robatzek

   Article

   Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

   Insert

   FLS2 (FLS2 Mustard Weed)
   

   Use

   Tags

   3xmyc

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528-AMOT(1-300)-sortase

   Plasmid

   #166706

   Purpose

   expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   AMOT aa 1-300
   

   Use

   Tags

   His-SUMO and Sortase tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-FLAG-BMAL1-S513A

   Plasmid

   #186831

   Purpose

   Expresses FLAG-BMAL1-S513A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 513 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-FLAG-BMAL1-S515A

   Plasmid

   #186832

   Purpose

   Expresses FLAG-BMAL1-S515A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 515 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-FLAG-BMAL1-S516A

   Plasmid

   #186833

   Purpose

   Expresses FLAG-BMAL1-S516A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 516 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-FLAG-BMAL1-S519A

   Plasmid

   #186834

   Purpose

   Expresses FLAG-BMAL1-S519A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 519 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-Flag-S1pr1-R120A

   Plasmid

   #203707

   Purpose

   Overexpression of FLAG-S1PR1-R120A mutant.

   Depositor

   Timothy Hla

   Insert

   Flag-S1PR1-R120A (S1PR1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R120A

   Promoter

   Available since

   Dec. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA-T-intron(Neo)-G(LNL)

    Plasmid

    #36908

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS-pac-T2A-iCre

    Plasmid

    #113849

    Purpose

    Puromycin resistance gene and iCre recombinase

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-GBP-dCas9-mRFP

    Plasmid

    #102933

    Purpose

    Expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

    Depositor

    Sebastian Bultmann

    Article

    Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GBP (GFP binding protein) and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pX330-Flag-eSpCas9-D10A

    Plasmid

    #80449

    Purpose

    enhanced SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Insert

    ehSpCas9_D10A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCASP-SptP167-3G124-HilA

    Plasmid

    #153322

    Purpose

    Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP167-3G124 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCASP-HilA empty vector

    Plasmid

    #153333

    Purpose

    Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Type

    Empty backbone

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSD_2xNLS-SpCas9MT3-NLS-FKBP_SL

    Plasmid

    #107305

    Purpose

    Expresses R1335K mutant SpCas9 fused to FKBP with extra NLS in mammalian cells

    Depositor

    Scot Wolfe

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2x NLS and FKBP

    Expression

    Mammalian

    Mutation

    R1335K and fused to FKBP

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS24: pCascade(PmcCAST)_entry

    Plasmid

    #168157

    Purpose

    Inducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCasper-pNos-PCP-eGFP

    Plasmid

    #180274

    Purpose

    to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

    Depositor

    Hernan Garcia

    Article

    Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

    Insert

    PCP-eGFP
    

    Use

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCASP-SptP120-3m160-HilA

    Plasmid

    #153325

    Purpose

    Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP120-3m160 DARPin
    

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-Trex2-bpA-EF1BFP

    Plasmid

    #111144

    Purpose

    Expression of mouse Trex2 and BFP reporter in mammalian cells

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Inserts

    mouse Trex2 coding region (Trex2 Mouse)
    BFP (Rnf112 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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