We narrowed to 53,271 results for: LIS;

1. pGEMT-PTE2A

   Plasmid

   #111773

   Purpose

   Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Type

   Empty backbone

   Use

   Cloning intermediate

   Promoter

   T7

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay-GRAB_eCBmut-IRES-mCherry-CAAX

   Plasmid

   #164612

   Purpose

   Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid non-responsive sensor GRAB_eCBmut
   

   Expression

   Mammalian

   Mutation

   S383T, F177A

   Promoter

   CMV

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. A&C-BEmax

   Plasmid

   #157947

   Purpose

   Mammalian expression of A&C-BEmax

   Depositor

   Dali Li

   Article

   Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   A&C-BEmax
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-puro-CMV-GC3AI

   Plasmid

   #78910

   Purpose

   Indicator of caspase-3-like protease activity

   Depositor

   Binghui Li

   Article

   Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

   Insert

   GC3AI
   

   Use

   Lentiviral

   Tags

   HA and Myc

   Mutation

   S320A, deletion of AA1-200

   Promoter

   CMV

   Available Since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-GRAB_ACh3.0

   Plasmid

   #121923

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

   Insert

   GPCR activation based ACh sensor GRAB-ACh3.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ACBE

   Plasmid

   #204607

   Purpose

   Mammalian expression of adenine-to-cytosine base editor ACBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. hyeA3A-BE4max

   Plasmid

   #157944

   Purpose

   Mammalian expression of hyeA3A-BE4max

   Depositor

   Dali Li

   Article

   Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

   Insert

   hyeA3A-BE4max
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N57G

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO-GRAB-rDA3m

   Plasmid

   #208708

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m in neurons in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3m
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hsyn-GRAB_rDA1m

   Plasmid

   #140556

   Purpose

   Expresses the red fluorescent medium-affinity DA sensor GRAB_rDA1m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   Red fluorescent DA sensor GRAB_rDA1m (medium affinity)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Td-CBEmax

    Plasmid

    #196600

    Purpose

    Mammalian expression of Td-CBEmax

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDisplay-GRAB_r5-HT1.0

    Plasmid

    #208713

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-GfaABC1D-GRAB_g5-HT3.0

    Plasmid

    #208727

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytes

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    GfaABC1D

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-GRAB_NE1h

    Plasmid

    #123309

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE sensor GRAB_NE1h
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hsyn-GRAB_ATP1.0mut

    Plasmid

    #167578

    Purpose

    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

    Plasmid

    #177130

    Purpose

    Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Mutation

    W428A/W377A (m6A binding defects), and siRNA resi…

    Promoter

    Ef1a

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. FlucWT-HA-GFP11-N1

    Plasmid

    #91954

    Purpose

    Expresses FlucWT-HA-GFP11 in mammalian cells

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    FlucWT-HA-GFP11
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. FlucDM-HA-GFP11-N1

    Plasmid

    #91956

    Purpose

    Expresses aggregation-prone FlucDM-HA-GFP11 in mammalian cells

    Depositor

    Rong Li

    Article

    Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

    Insert

    FlucDM-HA-GFP11
    

    Expression

    Mammalian

    Mutation

    R188Q,R261Q

    Promoter

    CMV

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-DIO-GRAB_g5-HT3.0

    Plasmid

    #208726

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTXBI-mClover-YTH domain

    Plasmid

    #177123

    Purpose

    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTH domain) (YTHDC1 Human)
    

    Tags

    Mxe GyrA intein-Chitin binding domain for protein…

    Expression

    Bacterial

    Mutation

    YTH domain only (IDR1 and IDR2 deletion)

    Promoter

    T7

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDisplay-GRAB_OT1.0

    Plasmid

    #185384

    Purpose

    Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    GPCR activation based oxytocin sensor GRAB_OT1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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