Addgene

We narrowed to 1,058 results for: agl

Showing: 341 - 360 of 1058 results

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pTol1-UAS:GtACR2-tdTomato

Plasmid

#124236

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR2-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)- e18

Plasmid

#131670

Purpose

Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1347

Plasmid

#154310

Purpose

Donor-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa Linker for CT slot

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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CALCOCO1-HA

Plasmid

#137766

Purpose

Expression and localization of tagged CALCOCO1

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 (CALCOCO1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_NPM1_G6

Plasmid

#178091

Purpose

Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

Plasmid

#156445

Purpose

For transient expression of mouse RAD21 (SCC1) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse RAD21 (Rad21 Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:CoChR-tdTomato

Plasmid

#124233

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

CoChR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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HA-GABARAP

Plasmid

#137760

Purpose

Expression of GABARAP

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAP (GABARAP Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-C-STAT1-mGFP-P2A-Puro

Plasmid

#199345

Purpose

express murine STAT1 protein fused with mGFP

Depositor

Massimo Mallardo

Article

Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

Insert

signal transducer and activator of transcription 1 (Stat1 Mouse)

Use

Lentiviral

Tags

mGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJW1836

Plasmid

#154337

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pPB CAG Otx2-IRES-EGFP

Plasmid

#153947

Purpose

piggyBac transposon vector with CAG promoter expressing Otx2 and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Otx2 (Otx2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAS133

Plasmid

#211504

Purpose

AAV genome to express mTagBF2 under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

mTagBF2

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol1-UAS:GtACR1-tdTomato

Plasmid

#124235

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR1 fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

GtACR1-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS134

Plasmid

#211505

Purpose

AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

Venus

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAS132

Plasmid

#211503

Purpose

AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Insert

mCherry

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEN765 -pCAGGS-3XFLAG-mKate2-(human)PDS5B-bpA

Plasmid

#156436

Purpose

For transient expression of human PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

human PDS5B (PDS5B Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5-rat ERK2-L73P/S151D

Plasmid

#40819

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

L73P, S151D

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-ASCL2

Plasmid

#133375

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

ASCL2 (ASCL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3C

Plasmid

#137758

Purpose

Expression of LC3C

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3C (MAP1LC3C Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-ChiNW-ASCL2

Plasmid

#133384

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

ASCL2 (ASCL2 Human)

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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