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hDAGLa-V5

Plasmid

#87676

Purpose

Expresses human DAGL? protein with intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Article

Singh et al Biosci Rep. 2016 May 6;36(3). pii: e0033

Insert

Human Diacylglycerol Lipase Alpha (DAGLA Human)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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hDAGLb-V5

Plasmid

#87677

Purpose

Expresses human DAGLb protein with intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Article

Singh et al Biochemistry. 2016 May 17;55(19):2713-21

Insert

Human Diacylglycerol Lipase Beta (DAGLB Human)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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HA-hDAGLa-V5

Plasmid

#87674

Purpose

Expresses human DAGLa protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Article

Zhou et al Mol Cell Neurosci. 2016 Oct;76:76-86. do

Insert

Human Diacylglycerol Lipase Alpha (DAGLA Human)

Use

Tags

HA and V5 tag

Expression

Mammalian

Mutation

Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

Promoter

T7

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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HA-hDAGLb-V5

Plasmid

#87675

Purpose

Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Insert

Human Diacylglycerol Lipase Beta (DAGLB Human)

Use

Tags

HA and V5 tag

Expression

Mammalian

Mutation

Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

Promoter

T7

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Use

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo

Plasmid

#221899

Purpose

Expresses alpha tubulin fused to mStayGold

Depositor

Janelia Integrative Imaging

Insert

mStayGold-aTubulin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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mito-mStayGold_pTwistCMVBetaGlobWPRENeo

Plasmid

#221898

Purpose

Expresses mStayGold targeted to the mitochondria

Depositor

Janelia Integrative Imaging

Insert

cox8-mStayGold

Use

Tags

Cox8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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Tagln2-GFP

Plasmid

#221530

Purpose

Fluorescently tagged Tagln2-GFP

Depositor

Alessandro Ori

Insert

Tagln2 (TAGLN2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_Ef1a-->flagLaM4_Notch_tTA

Plasmid

#162237

Purpose

Lentiviral vector for the expression of LaM 4 mCherry nanobody notch tTA: flagLaM4_Notch_tTA in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaM4 mCherry nanobody Notch tTA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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p2.1 (pAGL2A)

Plasmid

#27563

Depositor

Gregg Semenza

Article

Semenza et al J Biol Chem. 1996 Dec 20. 271(51):32529-

Insert

ENO1 promoter (ENO1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
Halo_aTubulin-pTwistCMVBetaGlobWPRENeo

Plasmid

#221901

Purpose

Expresses Halo7 fused to alpha tubulin

Depositor

Janelia Integrative Imaging

Insert

Halo7-aTubulin

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pQTEV-TAGLN2

Plasmid

#31569

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

transgelin 2 (TAGLN2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pgWIZ-SGCAgl

Plasmid

#131456

Purpose

Encodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) to cure LGMD2D and luciferase enzyme for in vivo live imaging

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

SGCA (SGCA Human)

Use

Tags

3xFLAG

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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T7-AglC-His6

Plasmid

#89726

Purpose

Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglC

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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T7-AglK-His

Plasmid

#89714

Purpose

Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglK

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Plagl2

Plasmid

#228061

Purpose

transiently express mouse Plagl2

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Plagl2 (Plagl2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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Lck-Halo_pTwistCMVBetaGlobWPRENeo

Plasmid

#221900

Purpose

Expresses Halo7 targeted to the plasma membrane

Depositor

Janelia Integrative Imaging

Insert

Lck-Halo7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CFP_ betaGlobin_24XPP7_24XMS2_Splicing_ reporter

Plasmid

#61762

Purpose

Dual Color betaGlobin reporter to study splicing kinetics in vivo

Depositor

Daniel Larson

Article

Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

Insert

b-Globin

Use

Tags

24 X MS2 hairpin cassette in 3'UTR of b-Glob…

Expression

Mammalian

Mutation

Promoter

Dox inducible Tet-ON promoter

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a_flagLaM4-Notch-Gal4VP64

Plasmid

#216669

Purpose

Expresses anti-mCherry synNotch Gal4VP64

Depositor

Leonardo Morsut

Insert

anti-mCherry synNotch Gal4VP64

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPROBE'-gfp[tagless]

Plasmid

#40165

Depositor

Steven Lindow

Article

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotor probe

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge