Addgene

We narrowed to 13,827 results for: nts

Showing: 341 - 360 of 13827 results

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pET28a(+)-TrxB2

Plasmid

#225741

Purpose

To express TrxB2(Rv3913) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxB2

Use

Tags

6xHis

Expression

Bacterial

Mutation

TrxB2

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelI

Plasmid

#225730

Purpose

To express MelI (Rv1939) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melI

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelK

Plasmid

#225732

Purpose

To express MelK (Rv1941) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melK

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelJ

Plasmid

#225731

Purpose

To express MelJ (Rv1940) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melJ

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pNTI743 dual-barcoded gRNA parent vector

Plasmid

#164915

Purpose

CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI733 pRPR1(TetO)-RPC31-sgRNA

Plasmid

#164913

Purpose

For yeast genomic integration of sgRNA against RPC31

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-RPC31-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI732 pRPR1(TetO)-HTS1-sgRNA

Plasmid

#164912

Purpose

For yeast genomic integration of sgRNA against HTS1

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-HTS1-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRPL28 CaURA3 (pNTI592)

Plasmid

#115431

Purpose

URA3-marked RPS28

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
URA3

Use

Tags

Expression

Yeast

Mutation

KpnI site in intron

Promoter

Ashbya gossypii and endogenous

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pBW1942_ hU6-PV2-lox2272-BbsI-ENTRY-gRNA

Plasmid

#89055

Purpose

PV2 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW1720_hU6-PV1-FRT-lox2272-BbsI-ENTRY-gRNA

Plasmid

#89053

Purpose

PV1 gRNA entry vector for CRISPR decoder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW769_pCAG-PV3-FRT-loxP-iRFP720-BGHpA

Plasmid

#87576

Purpose

PV3 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iRFP720

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW806_pCAG-PV3-FRT-loxP-GFP-BGHpA

Plasmid

#87582

Purpose

PV3 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBW807_pCAG-PV4-loxP-mCherry-BGHpA

Plasmid

#87583

Purpose

PV4 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW770_pCAG-PV4-loxP-mRuby2-BGHpA

Plasmid

#87577

Purpose

PV4 for 2-input, 4-output decoder circuit

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBW707_pCAG-PV3-FRT-loxP-mCherry

Plasmid

#87571

Purpose

PV3 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBW705_pCAG-PV1-FRT-lox2272-STOP

Plasmid

#87569

Purpose

PV1 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

Neomycin Resistance Gene

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBW706_pCAG-PV2-lox2272-GFP

Plasmid

#87570

Purpose

PV2 for 2-input, 2-output circuit library

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-Chaetomium thermophilum LIC

Plasmid

#74782

Purpose

encodes dynein light intermediate chain (LIC) from Chaetomium thermophilum; no tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Chaetomium thermophilum dynein light intermediate chain

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS-M1A(KaganE18)

Plasmid

#52009

Purpose

Expresses the human MAVS CDS containing the point mutation M1A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M142A(KaganE19)

Plasmid

#52010

Purpose

Expresses the human MAVS CDS containing the point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only