We narrowed to 1,626 results for: sp1

1. pEGFP-rUSP19ER-CAmut

   Plasmid

   #155249

   Purpose

   for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus

   Depositor

   Simon Wing

   Insert

   USP19 (ER isoform) CA mutant (Usp19 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cys(545) to Ala

   Promoter

   CMV

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-HisFlag-rUSP19CYT

   Plasmid

   #155238

   Purpose

   for mammalian overexpression of the cytoplasmic isoform of rat USP19

   Depositor

   Simon Wing

   Insert

   USP19 (Cytoplasmic isoform) (Usp19 Mouse, Rat)
   

   Use

   Tags

   6XHis-3XFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-Nsp15

   Plasmid

   #157720

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp15 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pcDNA5-FRT-TO-Nsp14

   Plasmid

   #157717

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pmiRGLO-CTDSP1-201

   Plasmid

   #117328

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR CTDSP1-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Xl-VSP1 R152Q

   Plasmid

   #51881

   Purpose

   expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available since

   May 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. NaVSp1 FL DeltaCTD pIRES

   Plasmid

   #128077

   Purpose

   Mammalian cell expression vector for NaVSp1 bacterial sodium channel. Residues 1-224.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NaVSp1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. NaVSp1 Ae1 CC pIRES

   Plasmid

   #128079

   Purpose

   Mammalian cell expression vector for (NaVSp1 1-236 NaVAe1 265-288) chimera bacterial sodium channel.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NaV Sp1 and NaVAe1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. NaVSp1 Ms CTD pIRES

   Plasmid

   #128081

   Purpose

   Mammalian cell expression vector for (NaVSp1 1-220 NaVMs 222-274) chimera bacterial sodium channel.

   Depositor

   Dan Minor

   Article

   Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

   Insert

   NavSp1_MsCTD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. NaVSp1 Pz CTD pIRES

    Plasmid

    #128083

    Purpose

    Mammalian cell expression vector for (NaVSp1 1-220 NaVPz 224-262) chimera bacterial sodium channel.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NavSp1_PzCTD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. HM3C NaVSp1 pET24b

    Plasmid

    #128088

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

    Depositor

    Dan Minor

    Article

    Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

    Insert

    NaVSp1p
    

    Use

    Tags

    3C Cleavage, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-Nsp14-TEV-Strep

    Plasmid

    #154953

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCDNA4 CoV-2 nsp1 3xFLAG

    Plasmid

    #176057

    Purpose

    Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG tag fusion to nsp1's C-terminus.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1(nsp8-nsp7)(nsp12)

    Plasmid

    #165451

    Purpose

    Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

    Depositor

    Marc Delarue

    Article

    Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

    Inserts

    nsp12
    nsp8
    nsp7
    

    Use

    Tags

    14Histidine-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS Sp1 full length

    Plasmid

    #12096

    Depositor

    Robert Tjian

    Article

    Kadonaga et al Cell. 1987 Dec 24. 51(6):1079-90.

    Insert

    Sp1 (SP1 Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pDONR223 SARS-CoV-2 NSP12

    Plasmid

    #141265

    Purpose

    Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pDONR223 SARS-CoV-2 NSP16

    Plasmid

    #141269

    Purpose

    Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDONR207 SARS-CoV-2 NSP1

    Plasmid

    #141255

    Purpose

    Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. MAC-C Sars-CoV-2 NSP12_P314L

    Plasmid

    #194813

    Purpose

    Sars-CoV-2 NSP12_P314L in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    NSP12_P314L (ORF1ab Sars-CoV-2)
    

    Use

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    P314L

    Promoter

    Available since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pET28-6His-SUMO-Nsp10

    Plasmid

    #154959

    Purpose

    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

    Depositor

    Justin Kollman

    Insert

    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    6His-Thrombin-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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