We narrowed to 4,857 results for: U6...

1. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

   Plasmid

   #138295

   Purpose

   gRNA for CRISPR/Cas9 knockout of human TRIM21

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   gRNA TRIM21 (TRIM21 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV ORANGE Gria1-HaloTag KI

   Plasmid

   #139655

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv1_SDHB

   Plasmid

   #177981

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHB (SDHB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP317-pAAV-U6SaCas9gRNA(SapI)-EFS-GFP- KASH-pA

   Plasmid

   #113694

   Purpose

   U6 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-ITR-PytR-mCherry

   Plasmid

   #204868

   Purpose

   Expresses pyrrolysyl tRNA and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. mazei pyrrolysyl tRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C

   Promoter

   U6

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-ITR-EcYtR-mCherry

   Plasmid

   #217362

   Purpose

   Expresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   E. coli tyrosine tRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX462-hPRKAA1-gRNA_B

   Plasmid

   #74375

   Purpose

   gRNA_B to knockout human AMPK alpha 1 using Cas9n

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR v2-sgDHODH-4

   Plasmid

   #186022

   Purpose

   knock out DHODH in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX462-hPRKAA1-gRNA_A

   Plasmid

   #74374

   Purpose

   gRNA_A to knockout human AMPK alpha 1 using Cas9n

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   human AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-COSMC-KO

    Plasmid

    #80008

    Purpose

    gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    COSMC (C1GALT1C1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-5'-aptamer-tagged-SVA-lncRNA-AK057321-mcherry

    Plasmid

    #202818

    Purpose

    Lentiviral vector to express 5'-aptamer tagged SVA-lncRNA AK057321 with CMV for mcherry expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    SVA-lncRNA AK057321
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    addition of a dual aptamer RNA tag 5' to the…

    Promoter

    U6

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCRISPR v2-sgDHODH-2

    Plasmid

    #186023

    Purpose

    knock out DHODH in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pORANGE GFP-Clta KI

    Plasmid

    #131483

    Purpose

    Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Clta Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. AllCB shRNA-1

    Plasmid

    #160065

    Purpose

    Knock Down all Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    mouse U6 promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR Human CASP9 -2

    Plasmid

    #198420

    Purpose

    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

    Depositor

    Hannah Rabinowich

    Article

    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

    Insert

    Human CASP9 Specific gRNA (CASP9 Human)
    

    Use

    CRISPR

    Tags

    Cas9/Orange Fluorescent Protein Reporter

    Expression

    Mammalian

    Mutation

    Promoter

    U6; CMV

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR Human CASP9 -1

    Plasmid

    #198419

    Purpose

    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

    Depositor

    Hannah Rabinowich

    Article

    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

    Insert

    Human CASP9 Specific gRNA (CASP9 Human)
    

    Use

    CRISPR

    Tags

    Cas9/Orange Fluorescent Protein Reporter

    Expression

    Mammalian

    Mutation

    Promoter

    U6; CMV

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ATP1A1_G4_Q118R_v2_Dual_epegRNA_tevopreQ1

    Plasmid

    #187456

    Purpose

    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4_v2 optimized epegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R_v2 epegRNA (tevopreQ1) (ATP1A1 Human)
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCRISPRv1_SDHA

    Plasmid

    #177980

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHA (SDHA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX459-CTNNB1-S45

    Plasmid

    #164587

    Purpose

    Expresses a gRNA that overlaps the S45 codon of human CTNNB1

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 gRNA (CTNNB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMKO.1-shCcnb2

    Plasmid

    #162794

    Purpose

    Ccnb2 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Ccnb2 shRNA (Ccnb2 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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