We narrowed to 9,936 results for: ada

1. SUN2delCC

   Plasmid

   #130836

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

   Insert

   SUN2
   

   Use

   Tags

   mCherry-FLAG-HA

   Expression

   Plant

   Mutation

   Deletion of coiled coil domain

   Promoter

   CaMV 35S

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. SUN2delSUNec

   Plasmid

   #130847

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

   Insert

   SUN2
   

   Use

   Gateway entry vector

   Tags

   mCherry-FLAG-HA

   Expression

   Mutation

   Deletion of SUN domain

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. SUN2delCCec

   Plasmid

   #130848

   Purpose

   Expression of plant LINC fluorescent fusion protein for cell biology

   Depositor

   Hank Bass

   Article

   Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

   Insert

   SUN2
   

   Use

   Gateway entry vector

   Tags

   mCherry-FLAG-HA

   Expression

   Mutation

   Deletion of coiled coil domain

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. Golden Gate TALEN and TAL effector kit 2.0

   Plasmid Kit

   #1000000024

   Purpose

   Used to efficiently assemble TALEN constructs with custom repeat arrays, containing anywhere between 12 and 31 repeats. Uses Golden Gate cloning method and has been validated in multiple organisms.

   Depositor

   Daniel Voytas, Adam Bogdanove

   Available since

   Jan. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

5. qTAG-AAVS1-Ef1a-Puro

   Plasmid

   #227266

   Purpose

   Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRE2-Bla(HA–RILP-FLAG)

   Plasmid

   #102424

   Purpose

   Dual tagged RILP for expression in mammalian cells.

   Depositor

   Steven Weinman

   Article

   Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   Rab interacting lysosomal protein (RILP Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-Responsive

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK419 (GAL-OsTIR1(F74G))

   Plasmid

   #140656

   Purpose

   GAL-OsTIR1(F74G)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLNHA-C1-HsXRN1

   Plasmid

   #66596

   Purpose

   expresses lambdaN-HA-tagged XRN1 in mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   XRN1 (XRN1 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-U6-crRNA-empty

   Plasmid

   #134921

   Purpose

   Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   crRNA for CRISPR-Cas3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS-spRYCas9-ABE-UMax

    Plasmid

    #222139

    Purpose

    Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    TadA-nSpRYCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-SNAP-PI(3,5)P2 Probe

    Plasmid

    #211512

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SnxA-2xPX
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. deltaNp63alpha-FLAG

    Plasmid

    #26979

    Depositor

    David Sidransky

    Article

    Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

    Insert

    ΔNP63alpha (TP63 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    dominant negative isoform of p63; aa 1–587

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK381 (AAVS1 CMV-OsTIR1F74G)

    Plasmid

    #140536

    Purpose

    AAVS1 CMV-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPR v2-sgLKB1 clone1

    Plasmid

    #162125

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-SNAP-PI(3,4,5)P3 Probe

    Plasmid

    #211514

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BTK1-PH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF5B-FRT-cilia-APEX

    Plasmid

    #73186

    Purpose

    Expresses cilia-APEX in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    cilia-APEX (Nphp3 Mouse, Glycine max)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. 6xHis-SNAP-PI(3,4)P2 Probe

    Plasmid

    #211511

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    TAPP1 cPHx3
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT3TS-spCas9-ABE-Umax-rest1

    Plasmid

    #222144

    Purpose

    Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLENTI_RPS3_Keima

    Plasmid

    #127140

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS3_Keima (RPS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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