Skip to main content
Addgene
Showing: 5431 - 5460 of 8427 results

  1. psLIB EF1alpha

    Plasmid
    #229954
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229954-plasmid-map-sequence-id-461280

    Enlarge

  2. psLIB TRE

    Plasmid
    #229955
    Purpose
    bigMamAct is evolution of biGBac for mammalian cells. Has empty Tet-responsive element (TRE)-driven cassette; users should clone gene of interest into shuttle plasmid prior to Gibson cloning
    Depositor
    Martin Pelosse
    Article
    Pelosse et al Synthetic Biology 2025.02.13
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    229955-plasmid-map-sequence-id-461253

    Enlarge

  4. pBH504

    Plasmid
    #215782
    Purpose
    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene
    Depositor
    Corey Wilson
    Article
    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:
    Inserts
    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Bt1311, P(O1), and P1
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215782-plasmid-map-sequence-id-430549

    Enlarge

  17. INT_Cargo_PgyrA_katG_dadA6

    Plasmid
    #212142
    Purpose
    Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212142-plasmid-map-sequence-id-420769

    Enlarge

  18. pBTK1054

    Plasmid
    #209544
    Purpose
    BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome at a neutral site
    Depositor
    Jeffrey Barrick
    Article
    Lariviere et al bioRxiv 2023.09.19.558440
    Insert
    Recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    209544-plasmid-map-sequence-id-419120

    Enlarge

Showing: 5431 - 5460 of 8427 results