We narrowed to 6,245 results for: Alk

1. S1 1029 CP ABEMax strategy 3

   Plasmid

   #135359

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1029 CP nSpyCas9 ABEMax
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. S1 1029 miniABEMax V82G strategy1

   Plasmid

   #135360

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   mini ABEMax 1029 nSpyCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. S2 1058 V82G ABEMax

   Plasmid

   #135361

   Purpose

   Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1058 nSpyCas9 V82G ABEMax
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. S1 1029 V82G ABEMax Strategy 3

   Plasmid

   #135363

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   1029 V82G ABEMax
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. miniABEMax V82G nSaCas9

   Plasmid

   #135365

   Purpose

   Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax V82G nSaCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AIDx-ID-202

   Plasmid

   #135344

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-202
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. FLAG-SENP2 NPm

   Plasmid

   #126593

   Purpose

   Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell lines

   Depositor

   Joanna Morris

   Article

   Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

   Insert

   SENP2 (SENP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-65 and L329A/L331A in t…

   Promoter

   CMV

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-SENP2 CCm

   Plasmid

   #126594

   Purpose

   Expresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian cells, Dox inducible in TetR cell lines

   Depositor

   Joanna Morris

   Article

   Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

   Insert

   SENP2 (SENP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 203-228 and siRNA resista…

   Promoter

   CMV

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 289aa Met133, 138, 186Ileu ELL2

   Plasmid

   #127272

   Purpose

   Expresses 289aa human ELL2 with Met133, 138, 186Ileu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met133, 138, 186 Ileu, cut with XbaI blunt fillin

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. frameshift ELL2

    Plasmid

    #127257

    Purpose

    For in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    XhoI site between the 1st &2nd Met was filled…

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. 564aa ELL2 bluescript

    Plasmid

    #127267

    Purpose

    For in vitro translation of human ELL2 lacking COOH region

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Addgene Plasmid 127256--SphI site filled in, then…

    Promoter

    T7

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIREN-RetroQ-HSPE-sh1

    Plasmid

    #92033

    Purpose

    pSIREN-RetroQ vector containing shRNA sequence to HPSE

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #1 to HPSE (Hpse Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pSIREN-RetroQ-HSPE-sh2

    Plasmid

    #92034

    Purpose

    pSIREN-RetroQ vector containing shRNA sequence to HPSE

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA #2 to HPSE (Hpse Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

    Plasmid

    #62579

    Purpose

    Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    SPRED1 (SPRED1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

15. LCV2_LacZ_sgRNA_2

    Plasmid

    #155093

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    LacZ_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5_mEGFP-EWS-FLI1

    Plasmid

    #237636

    Purpose

    For overexpression of mEGFP-EWS-FLI1

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EWS-FLI1 (EWSR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-mScarlet-Giantin

    Plasmid

    #196872

    Purpose

    Expresion of Giantin-fused to mScarlet. Used as Golgi marker

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mScarlet-Giantin (GOLGB1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Thy1.1-G5

    Plasmid

    #234839

    Purpose

    pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

    Depositor

    Gabriel Victora

    Article

    Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

    Insert

    Thy1.1 (Thy1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-P2A-5xGly

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28b-zDjCas13d-His

    Plasmid

    #234481

    Purpose

    Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    DjCas13d
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWZL hygro Flag HA TRAP220 wt

    Plasmid

    #17433

    Purpose

    Retroviral expression of Med1/TRAP220 in mammalian cells

    Depositor

    Kai Ge

    Article

    Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

    Insert

    TRAP220 (MED1 Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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