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pGW-FL-V5

Plasmid

#78858

Purpose

DULIP Prey Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Firefly luciferase, V5-tag

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGW-RL-PA

Plasmid

#78856

Purpose

DULIP Bait Destination Vector (Gateway, C-terminal)

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Type

Empty backbone

Use

Gateway destination vector

Tags

Renilla luciferase, Protein A

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[closed]

Plasmid

#71810

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[closed]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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BB3aN_14*

Plasmid

#98548

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-SGTAP no-pA

Plasmid

#82599

Purpose

3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

SBP-TEV-2x protein G

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR (GFP) cRel shRNA1

Plasmid

#22509

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

cRel shRNA1 (Rel Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-myc-BioID-C

Plasmid

#174737

Purpose

Overexpresion

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Type

Empty backbone

Use

Retroviral

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS4B

Plasmid

#145357

Purpose

Cell-free expression vector for Zika virus NS4B protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS4B

Tags

mCherry and 8xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_OXSR1_CCT

Plasmid

#178482

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

OXSR1_OXSR1 (OXSR1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM6-H9-mCherry

Plasmid

#66532

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H9

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H9

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-Lysosomes-20

Plasmid

#55495

Purpose

Localization: Lysosome Membrane, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

LAMP1

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pGEMT-Pax7bait-T2a-Cherry-pA

Plasmid

#111154

Purpose

knock in bait for Axolotl Pax7 locus

Depositor

Elly Tanaka

Article

Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;11

Insert

Pax7bait-T2a-Cherry-pA

Expression

Mammalian

Mutation

wt

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC

Plasmid

#71812

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSB74

Plasmid

#19591

Depositor

Jay Keasling

Article

Bang et al Appl Environ Microbiol. 2000 Sep . 66(9)

Insert

thiosulfate reductase (phsB S. enterica serovar Typhimurium LT2)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
pIE2-AD

Plasmid

#21603

Depositor

Takahiro Kusakabe

Article

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

NF-kappa-B p65 subunit (Rela Mouse)

Use

Gateway destination vector

Tags

Flag, Gateway destination cassette, and NLS

Expression

Insect

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_EYFP

Plasmid

#167890

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_EYFP

Plasmid

#167866

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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BB2_EF

Plasmid

#98538

Purpose

empty BB2 for transcription unit cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-long (pcDNA3.1+)

Plasmid

#75201

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNA500

Plasmid

#149641

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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