We narrowed to 5,112 results for: mcs

1. pVITRO1-SS-248

   Plasmid

   #68762

   Purpose

   ZF9-TF-V3 with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   ZF9-TF-V3
   ZF9-TF-V3
   mCherry
   

   Tags

   NLS and flag

   Expression

   Mammalian

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVITRO1-SS-115

   Plasmid

   #68730

   Purpose

   ZF2-TF with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   ZF2-VP64
   mCherry
   

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVITRO1-SS-117

   Plasmid

   #68732

   Purpose

   ZF4-TF with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   ZF4-VP64
   mCherry
   

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVITRO1-SS-128

   Plasmid

   #68734

   Purpose

   ZF6-TF with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   ZF6-VP64
   mCherry
   

   Tags

   NLS and flag

   Expression

   Mammalian

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVITRO1-SS-130

   Plasmid

   #68735

   Purpose

   ZF7-TF with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   ZF7-VP64
   mCherry
   

   Tags

   NLS and flag

   Expression

   Mammalian

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pVITRO1-SS-131

   Plasmid

   #68736

   Purpose

   ZF8-TF with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   ZF8-VP64
   mCherry
   

   Tags

   NLS and flag

   Expression

   Mammalian

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVITRO1-SS-132

   Plasmid

   #68729

   Purpose

   ZF1-TF with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   ZF1-VP64
   mCherry
   

   Tags

   FLAG and NLS

   Expression

   Mammalian

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-pcDNA3.1 Puro-CAG-Voltron2

   Plasmid

   #172909

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2
   

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

   Plasmid

   #179905

   Purpose

   Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

   Insert

   SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

   Promoter

   CAG

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pINTO-N3::hTET3 HxD

    Plasmid

    #136365

    Purpose

    Expresses epitope-tagged human TET3 catalytically dead (HxD mutation) in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Tags

    2xStrepTagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    HxD (H1077Y, D1079A)

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDNA5-FRT/TO-FLAG-TONSL

    Plasmid

    #221011

    Purpose

    mammalian expression vector of Flag tagged TONSL siRNA resistant

    Depositor

    Daniel Durocher

    Article

    O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

    Insert

    TONSL (TONSL Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

    Promoter

    CMV

    Available Since

    June 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pJT230 lenti pEF-rTetR(SE-G72P)-3XFLAG-NCOA3-TAD-T2A-mCherry-BSD-WPRE

    Plasmid

    #188763

    Purpose

    Lentiviral backbone containing NCOA3 as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    NCOA3 (NCOA3 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

    Plasmid

    #156415

    Purpose

    kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    mTagBFP2

    Mutation

    mutant KRAS4A-G12D

    Promoter

    kdrl

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHIS-MDH1-HIS

    Plasmid

    #184558

    Purpose

    Bacterial expression of human MDH1 with HIS Tag

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Malate dehydrogenase 1 (MDH1 Human)
    

    Tags

    6xHIS

    Expression

    Bacterial

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)

    Plasmid

    #180468

    Purpose

    kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    KRAS4A (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    V5-mScarlet-I

    Promoter

    kdrl

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. t1-405(del37/GAG)

    Plasmid

    #166129

    Purpose

    Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    T1head1-405(del37/GAG) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    aa1-405, with 37 aa deletion in the F1-loop which…

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

    Plasmid

    #156414

    Purpose

    kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4B-G12V (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Mutation

    mutant KRAS4B-G12V

    Promoter

    kdrl

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT222 lenti pEF-rTetR(SE-G72P)-3XFLAG-FOXO3 FOXO-TAD-T2A-mCherry-BSD-WPRE

    Plasmid

    #188760

    Purpose

    Lentiviral backbone containing FOXO3 FOXO-TAD as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    FOXO3 FOXO-TAD (FOXO3 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT226 lenti pEF-rTetR(SE-G72P)-3XFLAG-SMARAC2 QLQ-T2A-mCherry-BSD-WPRE

    Plasmid

    #188761

    Purpose

    Lentiviral backbone containing SMARCA2 QLQ as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    SMARCA2 QLQ (SMARCA2 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT228 lenti pEF-rTetR(SE-G72P)-3XFLAG-KIBRA WW-T2A-mCherry-BSD-WPRE

    Plasmid

    #188762

    Purpose

    Lentiviral backbone containing KIBRA WW as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    KIBRA WW (WWC1 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge