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pTEI122

Plasmid

#126062

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

ccdB Cassette

Use

Synthetic Biology; Module 0

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-fugu SLC2A4 promoter

Plasmid

#65101

Purpose

Luciferase reporter construct under the fugu (Takifugu rubripes) SLC2A4 promoter.

Depositor

Josep Planas

Article

Marin-Juez et al PLoS One. 2013 Nov 18;8(11):e80628. doi:

Insert

SLC2A4 promoter

Use

Luciferase

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR sgFTCD_1

Plasmid

#102312

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_1 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgFTCD_2

Plasmid

#102313

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_2 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgHAL

Plasmid

#102315

Purpose

genetic depletion of HAL

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgHAL (HAL Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-LKB1(S431A)

Plasmid

#84641

Purpose

mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(S431A) (STK11 Synthetic, Human)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

PKA phosphorylation site Serine 428 mutated to Al…

Promoter

CMV

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pWP1_mHAL

Plasmid

#102318

Purpose

expression of mouse cDNA of HAL refractory to the guide in plasmid 4

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Hal (Hal Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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GST-AR-4-52

Plasmid

#104199

Purpose

Expresses human AR-4-52 in in pGEX-4T-1 as GST fusion protein

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

human androgen receptor aa 4-52 (AR Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52_S1966C

Plasmid

#98681

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966C

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

S1966C

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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hFlt1 V767A

Plasmid

#86038

Purpose

human VEGFR1 with V to A mutation at position 767

Depositor

Christie Thomas

Article

Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

Insert

human Flt1 V767A (FLT1 Human)

Tags

FLAG, HA (please see depositor comments below), a…

Expression

Mammalian

Mutation

V767A

Promoter

CMV

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch12_62418577-gRNA-rev

Plasmid

#81217

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch12 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch12_62418577-gRNA-for

Plasmid

#81216

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch12 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCis_2+5

Plasmid

#60586

Purpose

a 2-integrase plasmid

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cis_2+5

Expression

Bacterial

Promoter

pBAD

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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AI-CN590

Plasmid

#62596

Purpose

KCTD13 N-terminus disruption by homolgy-directed repair

Depositor

Joshua Grimley

Article

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Insert

Cerulean-P2A-PuroR (KCTD13 Human)

Use

TALEN; Homology-directed repair donor

Expression

Mammalian

Promoter

hEF1alpha

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

Plasmid

#52897

Purpose

Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Ssk1t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
1208 p3e-long etv2 3’UTR

Plasmid

#49010

Purpose

Zebrafish Long length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

Longest 3' UTR

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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800 pME-Bozozok

Plasmid

#49008

Purpose

full length coding sequence for zebrafish bozozok gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

dharma (dharma )

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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592 p3e-EST etv2 3’UTR

Plasmid

#49003

Purpose

Zebrafish EST annotated length etv2 3' UTR in gateway 3' Entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway 3' entry

Mutation

only the 3' UTR

Promoter

3' gateway entry vector

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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PJ14

Plasmid

#25912

Depositor

Kenneth Marcu

Article

Lang et al Nucleic Acids Res. 1982 Jan 22. 10(2):61

Insert

Ig germline mu switch region

Expression

Bacterial

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
pBlue-hFX-HA

Plasmid

#14987

Depositor

Michael Ristow

Article

Shoichet et al Hum Mol Genet. 2002 Apr 1. 11(7):815-21.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Bacterial

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only