We narrowed to 30,244 results for: PLE

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pRec-TreR

Plasmid

#104574

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI-TreR chimera constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR, lacI-treR

Use

Synthetic Biology

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pAWp9-2

Plasmid

#73853

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_R4cons_Y129H

Plasmid

#64302

Purpose

Conservative version of OmpA Redesign 4 with Y129H mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_Y129H

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

25 mutations to lipid-facing surface

Promoter

T7

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only
Lenti-dCas9-KRAB-blast

Plasmid

#89567

Purpose

Plasmid expression dCas9 protein in fusion with KRAB domain

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

dCas9

Use

Lentiviral

Tags

Blasticidin resistance gene and KRAB

Mutation

D10A and H840A

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMK232 (CMV-OsTIR1-PURO)

Plasmid

#72834

Purpose

Expresses OsTIR1 under the control of the CMV promoter

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

AAVS1-CMV-OsTIR1-PURO-AAVS1

Expression

Mammalian

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pTP1112

Plasmid

#104158

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTP1122

Plasmid

#104159

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-mCh-Cry2WT

Plasmid

#101221

Purpose

Blue-light dependent optogenetic protein Cry2

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2PHR

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pOlyA(WT)

Plasmid

#132391

Purpose

Ostreolysin A for sensing sphingomyelin/cholesterol complexes

Depositor

Arun Radhakrishnan

Article

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A wild-type

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, C94S, S151C

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pTP955

Plasmid

#104164

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pHSV-TK

Plasmid

#165801

Purpose

Minimal viral terminator derived from Herpes Simplex 1 thymidine kinase gene

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

HSV-TK

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbC-dsRED

Plasmid

#84034

Purpose

Contains Golden Gate cassette to express up to four gRNAs and dsRED

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pX330S-3

Plasmid

#58779

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDAC627

Plasmid

#195240

Purpose

Mammalian expression of Csm complex from separate promoters; Puro backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; Puro

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2

Plasmid

#88917

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pTOX8

Plasmid

#127453

Purpose

Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
tsPurple chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

apFAB46 and pRha

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pX330S-4

Plasmid

#58780

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Cyto-V5-APEX2

Plasmid

#170572

Purpose

Expresses APEX2 in cytosol

Depositor

James Olzmann

Article

Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

Insert

APEX2

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDAC435

Plasmid

#195238

Purpose

Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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