We narrowed to 5,142 results for: mos

1. CIRTS-Gas5

   Plasmid

   #213172

   Purpose

   CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

   Insert

   CIRTS-Calm3-U6-Gas5 gRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Syn1, U6

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

   Plasmid

   #229839

   Purpose

   A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. GABPA_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176970

   Purpose

   Mammalian expression vector encoding GABPA and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GABPA (GABPA Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC-SUR-YR

   Plasmid

   #61768

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Sur gene
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Promoter

   Magnaporthe oryzae ILV promoter

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. GABPA_pLENTI-CAG-IRES-GFP

   Plasmid

   #176999

   Purpose

   Mammalian lentiviral expression vector encoding GABPA

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GABPA (GABPA Human)
   

   Use

   Lentiviral

   Promoter

   CAG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEos3.2-Clathrin-15

   Plasmid

   #57452

   Purpose

   Localization: Clathrin Vesicles, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Article

   Huang et al Nat Methods. 2013 Jul;10(7):653-8. doi:

   Insert

   Clathrin (CLTB Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

   Plasmid

   #188771

   Purpose

   dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9-XTEN80-KRAB(Kox1)
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Promoter

   EFS

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

   Plasmid

   #218914

   Purpose

   PiggyBac transposition

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )
   

   Expression

   Mammalian

   Mutation

   2363G>C and 4330T>C

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-B103 (LM2963)

   Plasmid

   #208960

   Purpose

   A variant CE1 construct with B103 DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-B103-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

    Plasmid

    #208953

    Purpose

    A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEos3.2-EB3-7

    Plasmid

    #57454

    Purpose

    Localization: MT End Binding Protein, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Article

    Huang et al Nat Methods. 2013 Jul;10(7):653-8. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid

    #208944

    Purpose

    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEmerald-EB3-7

    Plasmid

    #54075

    Purpose

    Localization: MT End Binding Protein, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Huang et al Nat Methods. 2013 Jul;10(7):653-8. doi:

    Insert

    EB3 (MAPRE3 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. GLI1_pLENTI-CAG-IRES-GFP

    Plasmid

    #176992

    Purpose

    Mammalian lentiviral expression vector encoding GLI1

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. GLI1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176968

    Purpose

    Mammalian expression vector encoding GLI1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GLI1 (GLI1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

    Plasmid

    #208962

    Purpose

    Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

    Plasmid

    #208955

    Purpose

    A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-iStat3-BFP

    Plasmid

    #216870

    Purpose

    Lenti-iStat3-BFP plasmid contains the TRE (doxycycline inducible) promoter followed by the Stat3 mouse cDNA, a T2A and a Blue Fluorescent Protein (BFP) sequence.

    Depositor

    Bernhard Payer

    Article

    Barrero et al Sci Adv. 2024 Aug 9;10(32):eadj8862. doi

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A-TagBFP

    Expression

    Mammalian

    Promoter

    Tetracycline-Response-Element (TRE)

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. H14-MBP-SUMO-FLAG-LEM

    Plasmid

    #101778

    Purpose

    Expresses His-MBP-tagged mutant LEM-domain (FLAG-tagged) in E.coli (SUMO-cleavable)

    Depositor

    Daniel Gerlich

    Article

    Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

    Insert

    MAN1 (LEMD3 Human)
    

    Tags

    His14-MBP-SUMO-FLAG

    Expression

    Bacterial

    Mutation

    amino acids 6-51 of human MAN1 (LEM domain)

    Promoter

    T5

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)

    Plasmid

    #208972

    Purpose

    Recruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    ePhi29(+exo)-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge