We narrowed to 6,241 results for: nls

1. pdCas9-NED

   Plasmid

   #109358

   Purpose

   Mammalian vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   dCas9, SV40 NLS, 3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Thy1-Brainbow-2.1 R

   Plasmid

   #18727

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. M-NMcas

   Plasmid

   #48670

   Purpose

   Mammalian N. meningitidis Cas9 expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   NLS

   Promoter

   CMV

   Available Since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

   Plasmid

   #174137

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

   Promoter

   CMV and T7

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_dCas9_VP64

   Plasmid

   #49015

   Purpose

   encodes human-optimized dCas9_VP64 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   dCas9_VP64 (human-codon-optimized)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1394

   Plasmid

   #129522

   Purpose

   pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   SpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   3XFLAG-NLS

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL358

   Plasmid

   #169147

   Purpose

   Encodes OptoQ-AMP1

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMAL513

   Plasmid

   #169153

   Purpose

   Encodes OptoQ-INVRT1

   Depositor

   Jose Avalos

   Article

   Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

   Insert

   VP16-EL222
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mini-donor - NKX2.5-nEGFP

   Plasmid

   #209287

   Purpose

   this mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locus

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

   Insert

   EGFP-nls
   

   Use

   Knockin donor vector

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available Since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAL728

    Plasmid

    #169152

    Purpose

    Encodes OptoQ-AMP6

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR1056

    Plasmid

    #71824

    Purpose

    Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_6xHis-MBP-TEV-SPydCas9 (D10A,H840A)-2xNLS
    

    Use

    CRISPR

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. M-SPcas

    Plasmid

    #48668

    Purpose

    Mammalian S. pyogenes Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAL498

    Plasmid

    #169154

    Purpose

    Encodes OptoQ-INVRT4

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR1054

    Plasmid

    #71822

    Purpose

    Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
    

    Use

    CRISPR

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMAL724

    Plasmid

    #169151

    Purpose

    Encodes OptoQ-AMP5

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. rtTA and TagBFP cassettes

    Plasmid

    #62452

    Purpose

    MXS_chaining vector with 2 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::NLS-TagBFPx3-bGHpA
    CMV::rtTA3-bGHpA
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid

    #174140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMAL669

    Plasmid

    #169148

    Purpose

    Encodes OptoQ-AMP2

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMAL722

    Plasmid

    #169149

    Purpose

    Encodes OptoQ-AMP3

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge