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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,984 results for: nls

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Showing: 3701 - 3720 of 11984 results

  2. PB-NLS-ZapCV2-ALA

    Plasmid
    #203665
    Purpose
    Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells
    Depositor
    Amy Palmer
    Publication
    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZapCV2-Ala zinc dead sensor
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Cys and His in Zap1 is mutated with Ala
    Promoter
    CMV promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203665-plasmid-map-sequence-id-402878

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  4. pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

    Plasmid
    #133789
    Purpose
    CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CjeCas9
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CAG
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133789-plasmid-map-sequence-id-268569

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  6. PB-NLS-ZapCV2-ALA

    Plasmid
    #203665
    Purpose
    Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells
    Depositor
    Amy Palmer
    Publication
    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZapCV2-Ala zinc dead sensor
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Cys and His in Zap1 is mutated with Ala
    Promoter
    CMV promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    203665-plasmid-map-sequence-id-402878

    Enlarge

  16. ARB342

    Plasmid
    #124025
    Purpose
    Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mAzamiGreen
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LS-A4_EF1a-NLS::mAzamiGreen-bgPA_R1
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124025-plasmid-map-sequence-id-252473

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  17. ARB343

    Plasmid
    #124026
    Purpose
    Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear mRuby2
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LS-A4_EF1a-NLS::mRuby2-bgPA_R1
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124026-plasmid-map-sequence-id-252475

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  19. NLS-4LNDDE488Q-pCDNA3.1(+)

    Plasmid
    #172314
    Purpose
    Expresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)
    Depositor
    Joshua Rosenthal
    Publication
    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lambda N peptide - human ADAR2 Deaminase Domain E488Q
    Tags
    6xHis, Flag, and Nuclear Localization Signal of S…
    Expression
    Mammalian
    Mutation
    changed the Glutamic acid 488 in the Deaminase Do…
    Promoter
    CMV
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172314-plasmid-map-sequence-id-339830

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Showing: 3701 - 3720 of 11984 results