We narrowed to 10,554 results for: aga

1. pVk7-33G1-Flag

   Plasmid

   #58950

   Purpose

   backbone for mouse recombinant antibody with added C-terminal FLAG tag

   Depositor

   Gavin Wright

   Article

   Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

   Type

   Empty backbone

   Use

   Tags

   FLAG tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

   Plasmid

   #179004

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-G-GECO1.1

   Plasmid

   #32445

   Purpose

   Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

   Promoter

   CMV

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK279 (mClover-Bsr)

   Plasmid

   #72795

   Purpose

   C-terminal tagging with mClover using CRIPSR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mClover-Bsr
   

   Use

   CRISPR

   Tags

   mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCK052 p5E exorh:mCherry

   Plasmid

   #195953

   Purpose

   Gateway p5E vector with reporter

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   exorh:mCherry
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-mNeonGreen-WPRE

   Plasmid

   #193341

   Purpose

   AAV-mediated expression of mNeonGreen under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBMN-AS-HNF4α

   Plasmid

   #139305

   Purpose

   Knocking down of human HNF4α through shRNA and amiRNA targeting HNF4α

   Depositor

   Claes Wadelius

   Article

   Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

   Insert

   shRNA and amiRNA against HNF4α
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SLF045: AAV-hSyn-eTsChR-GFP

   Plasmid

   #112280

   Purpose

   AAV based broad expression of eTsChR in neurons

   Depositor

   Adam Cohen

   Article

   Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

   Insert

   eTsChR
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. hSTARR-seq_SCP1 vector

   Plasmid

   #99292

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCold-TF-hPLEKHM1

    Plasmid

    #64146

    Purpose

    For protein expression in E.coli

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Use

    Tags

    6xHis-tag and Trigger Factor (TF)

    Expression

    Bacterial

    Mutation

    Promoter

    cspA

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pEYFP-Sec24D

    Plasmid

    #66614

    Purpose

    YFP fusion of Sec24D

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24D (SEC24D Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

    Plasmid

    #83947

    Purpose

    gRNA cassette-carrying vector with natMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    gRNA targeting ADE2 gene
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-tdKatushka2-WPRE

    Plasmid

    #193344

    Purpose

    AAV-mediated expression of tdKatushka2 under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    tdKatushka2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2ADW-lox-tdKeima-NES-lox-6011nls(ERK)-P2Av3-RGECO1

    Plasmid

    #162486

    Purpose

    For generating transgenic mice expressing ERK biosensor and RGECO1

    Depositor

    Michiyuki Matsuda

    Article

    Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

    Insert

    ERK biosensor based on FRET, and RGECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pATLIC

    Plasmid

    #110287

    Purpose

    A backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)

    Depositor

    In-Geol Choi

    Article

    Ko et al Appl Environ Microbiol. 2012 May;78(9):3

    Insert

    yfaL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCNL-N1_vinculin

    Plasmid

    #65691

    Purpose

    Expresses Vinculin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2

    Plasmid

    #87694

    Purpose

    Express ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    ERT2-PhoCl-Cre-PhoCl-ERT2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. 768 pME mApple-CAAX

    Plasmid

    #195961

    Purpose

    Gateway pME fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mApple-CAAX (membrane)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGF-mSox2-ONL

    Plasmid

    #65719

    Purpose

    mSox2 reporter plasmid (expresses ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    murine Sox2 promoter and ONL
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVk7-33G1-BioLHis

    Plasmid

    #58949

    Purpose

    backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

    Depositor

    Gavin Wright

    Article

    Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

    Type

    Empty backbone

    Use

    Tags

    6His tag and biotinylation peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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