We narrowed to 5,339 results for: pcdna3.1

1. pcDNA3-HCMVgH (TB40/E)

   Plasmid

   #136446

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FL-mCherry

   Plasmid

   #78862

   Purpose

   DULIP negative control (prey) to exclude unspecific background binding

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   mCherry
   

   Use

   Expression vector

   Tags

   Firefly luciferase, V5-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-FLAG-Bbs4

   Plasmid

   #128868

   Purpose

   For mammalian expression of mouse Bbs4

   Depositor

   Aimin Liu

   Article

   Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

   Insert

   Bbs4 (Bbs4 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Smac(1-60)mCherry

   Plasmid

   #40880

   Depositor

   Douglas Green

   Article

   Tait et al Dev Cell. 2010 May 18;18(5):802-13.

   Insert

   Smac(1-60)mCherry (DIABLO Human)
   

   Tags

   mCherry

   Promoter

   CMV

   Available Since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA-NLS-PhoCl-mCherry

   Plasmid

   #87691

   Purpose

   Express NLS-PhoCl-mCherry in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NLS-PhoCl-mCherry
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

   Plasmid

   #169533

   Purpose

   Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven ER-localized monomeric red fluorescent protein mRuby2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-CBS (WT)

   Plasmid

   #182923

   Purpose

   Mammalian expression plasmid for human CBS

   Depositor

   Philip Eaton

   Article

   Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

   Insert

   Cystathionine Beta-Synthase (CBS Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mfn2(K109A)-16xmyc

   Plasmid

   #26051

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Mfn2 (Mfn2 Mouse)
   

   Tags

   16xMyc

   Expression

   Mammalian

   Mutation

   K109A GTPase mutant

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

9. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

   Plasmid

   #227245

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1α and pTRE3G

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCD/NL-BH∆1

    Plasmid

    #41791

    Purpose

    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    HIV-1 Gag/Pol and RRE sequences
    

    Expression

    Mammalian

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFH2.30_Gag-MCP-dpol-T2A-eGFP

    Plasmid

    #205538

    Purpose

    Express HIV Gag-MCP-dpol-T2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    HIV Gag-MCP-dpol
    

    Expression

    Mammalian

    Mutation

    pol frameshift sequence recoded

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Opto-D2R

    Plasmid

    #106068

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-D2R
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL2783 (pTnsC-VP64_Vch)

    Plasmid

    #200896

    Purpose

    Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsC-VP64
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-NP

    Plasmid

    #74737

    Purpose

    mammalian expression of the Nucleoprotein of the A/New Caledonia/20/99 (H1N1) Influenza virus

    Depositor

    Elisa Vicenzi

    Article

    Di Pietro et al J Virol. 2013 Apr;87(8):4523-33. doi: 10

    Insert

    Nucleoprotein
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

    Plasmid

    #188994

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

    Plasmid

    #100279

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    Mutated catalytic domain (1025aa-End) (please see…

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Golgin-84-TEV #709#243

    Plasmid

    #42108

    Depositor

    Ayano Satoh

    Article

    Koreishi et al Antibody Technology Journal. 2011;1:5-11

    Insert

    Golgin-84 (GOLGA5 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    inserted ENLYFQG b/w 300 and 301

    Promoter

    CMV

    Available Since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pmyc-GFP-TNRC6A-(925-1709)

    Plasmid

    #42004

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa925-1709

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmyc-GFP-TNRC6A-(1-924)

    Plasmid

    #42003

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1-924

    Promoter

    CMV

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. YFP Gamma 8

    Plasmid

    #36106

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 8 (GNG8 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only