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pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86024

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Publication

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain: Changed aspartic acid 63…

Promoter

CMV IE

Available since

April 14, 2018

Availability

Academic Institutions and Nonprofits only

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pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Publication

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-His-TEV-DOT1L-MM

Plasmid

#89611

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

Depositor

Leemor Joshua-Tor

Publication

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L VVEL293MM Mutant (DOT1L Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGEN6-Ubc5 Sc

Plasmid

#83234

Purpose

pHis-par2-UbHs/Ubc5Sc/ Uba1Ta

Depositor

Gali Prag

Publication

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Inserts

Ubiqutin (UBC Human)
Ubc 5 (UBC5 Budding Yeast)
Uba 1

Tags

His

Expression

Bacterial

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pVP096

Plasmid

#222043

Purpose

pGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.

Depositor

Wayne Parrott

Publication

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

mRFP1e

Use

Synthetic Biology; Greengate compatible cloning v…

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pET28-a(+)-6xHis-mScarlet-I(+5)

Plasmid

#219418

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Publication

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mScarlet-I(+5)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

D125K, D186K, E216K, E238K

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_EGFR-NTEV-tcs-GV-2xHA

Plasmid

#214610

Purpose

EGFR receptor for split TEV assay

Depositor

Michael Wehr

Publication

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

EGFR-NTEV (EGFR Human)

Tags

TEV-Gal4-VP16-2xHA

Expression

Mammalian

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTag4C_SP-HTR2A-V2R-NTEV-tcs-GV-2xHA

Plasmid

#214615

Purpose

HTR2A receptor for split TEV assay

Depositor

Michael Wehr

Publication

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

HTR2A-NTEV (HTR2A Human)

Tags

TEV-Gal4-VP16-2xHA

Expression

Mammalian

Promoter

CMV

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCH117

Plasmid

#72320

Purpose

Pcin/tet-w reporter plasmid

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Type

Empty backbone

Expression

Bacterial

Promoter

Pcin/tet-w

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCH188

Plasmid

#72319

Purpose

Pcin/lac-m reporter plasmid

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Type

Empty backbone

Expression

Bacterial

Promoter

Pcin/lac-m

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCH189

Plasmid

#72321

Purpose

Pcin/tet-m reporter plasmid

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Type

Empty backbone

Expression

Bacterial

Promoter

Pcin/tet-m

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCH116

Plasmid

#72318

Purpose

Pcin/lac-w reporter plasmid

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Type

Empty backbone

Expression

Bacterial

Promoter

Pcin/lac-w

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCH101

Plasmid

#72308

Purpose

Prhl/lac-w reporter plasmid

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

modified YFP

Expression

Bacterial

Mutation

T203Y sfGFP->YFP

Promoter

Prhl/lac-w

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-Cx43-FS

Plasmid

#65440

Purpose

Cloning, T7 RNA expression

Depositor

Trond Aasen

Publication

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

T7 in vitro rna production

Expression

Bacterial

Mutation

Frameshift (FS) mutation 35delA

Promoter

T7

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S69A/S334A

Plasmid

#51588

Purpose

expresses RNAiR myc-tagged Rem2 non-14-3-3 interacting

Depositor

Suzanne Paradis

Publication

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

S69A and S334A; silent mutations at shRNA 1 and 2…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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XE15 Xbhh-C

Plasmid

#16995

Depositor

Randall Moon

Publication

Lai et al Development. 1995 Aug;121(8):2349-60. do

Insert

Xbhh-C (ihh Frog)

Use

Xenopus expression

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
XE14 Xbhh-N

Plasmid

#16994

Depositor

Randall Moon

Publication

Lai et al Development. 1995 Aug;121(8):2349-60. do

Insert

Xbhh-N (ihh Frog)

Use

Xenopus expression

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
XE13 Xbhh

Plasmid

#16993

Depositor

Randall Moon

Publication

Lai et al Development. 1995 Aug;121(8):2349-60. do

Insert

Xbhh (ihh Frog)

Use

Xenopus expression

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
CY027

Bacterial strain

#72402

Purpose

Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlR

Depositor

Matthew Bennett

Publication

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Bacterial resistance

None

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only
pAAV hSyn INTRON mCherry-Jph3 WPRE

Plasmid

#236237

Purpose

AAV expression of human Junctophilin3 N-terminally tagged with mCherry

Depositor

Jennifer Lippincott-Schwartz

Publication

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

Junctophilin 3 (JPH3 Human)

Use

AAV

Tags

mCherry

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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