Skip to main content
Addgene
Showing: 361 - 380 of 2024 results

  3. pRDA_526

    Plasmid
    #184859
    Purpose
    Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Feb. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    184859-plasmid-map-sequence-id-362663

    Enlarge

  11. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164559-plasmid-map-sequence-id-330205

    Enlarge

  12. pXPR_070

    Plasmid
    #164265
    Purpose
    Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164265-plasmid-map-sequence-id-330127

    Enlarge

  13. pXPR_071

    Plasmid
    #164558
    Purpose
    Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164558-plasmid-map-sequence-id-330194

    Enlarge

  14. pXPR_068

    Plasmid
    #164555
    Purpose
    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164555-plasmid-map-sequence-id-330129

    Enlarge

  15. pXPR_220

    Plasmid
    #164560
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164560-plasmid-map-sequence-id-330240

    Enlarge

  16. pcDNA3.1-ccdB-V5

    Plasmid
    #214985
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214985-plasmid-map-sequence-id-440020

    Enlarge

  17. pcDNA3.1-ccdB-3xHA

    Plasmid
    #214986
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214986-plasmid-map-sequence-id-440021

    Enlarge

  18. pcDNA3.1-ccdB-3xFLAG

    Plasmid
    #214984
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214984-plasmid-map-sequence-id-440018

    Enlarge

  19. pcDNA3.1-ccdB-TwinStrep

    Plasmid
    #214988
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Tags
    TwinStrep
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214988-plasmid-map-sequence-id-440022

    Enlarge

  20. pHAGE-APEX2-nDEST

    Plasmid
    #214992
    Purpose
    Gateway compatible empty vector (lentiviral/mammalian expression) with an N-terminal APEX2 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Flag-APEX2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    214992-plasmid-map-sequence-id-443329

    Enlarge

Showing: 361 - 380 of 2024 results