Skip to main content
Addgene
Showing: 361 - 380 of 622 results

  1. pSCALPs-HA-NFAT1 (4-460)-GFP

    Plasmid
    #88879
    Purpose
    Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP
    Depositor
    Silvia Monticelli
    Article
    Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi
    Insert
    HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
    Use
    Lentiviral
    Tags
    GFP fusion protein and HA tag
    Expression
    Mammalian
    Mutation
    amino acids 4-460 only; L78P & L287P *see bel…
    Promoter
    SFFV (spleen focus forming virus)
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    88879-plasmid-map-sequence-id-177202

    Enlarge

  5. MZB160

    Plasmid
    #217841
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    Arf1 (ARF1 Human)
    Use
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    L8K, Q71L
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217841-plasmid-map-sequence-id-432998

    Enlarge

  6. MZB224

    Plasmid
    #226998
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    Arf1 (ARF1 Human)
    Use
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    T31N
    Promoter
    T7
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    226998-plasmid-map-sequence-id-455977

    Enlarge

  9. pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

    Plasmid
    #186968
    Purpose
    Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.
    Depositor
    Brian Liau
    Article
    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d
    Insert
    pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
    Use
    Lentiviral
    Tags
    rTetR fusion
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    186968-plasmid-map-sequence-id-370475

    Enlarge

  12. MZB131

    Plasmid
    #217839
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    AP3D1 (AP3D1 Human)
    Use
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Deleted residues 1-919
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217839-plasmid-map-sequence-id-432995

    Enlarge

  13. MZB150

    Plasmid
    #217840
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (52-252) of Cytohesin-2 (CYTH2/ARNO) for rapid nucleotide exchange and activation of Arf GTPases
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    ARNO (CYTH2 Human)
    Use
    Tags
    10x His and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    Deleted residues 1-51 and 253-400
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217840-plasmid-map-sequence-id-432997

    Enlarge

  17. TFORF1777

    Plasmid
    #144310
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WHSC1 (NSD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    144310-plasmid-map-sequence-id-471158

    Enlarge

  18. TFORF1778

    Plasmid
    #142851
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    WHSC1 (NSD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    142851-plasmid-map-sequence-id-392726

    Enlarge

Showing: 361 - 380 of 622 results