We narrowed to 4,315 results for: Pet2

1. pET28a-AncMEcoGII291

   Plasmid

   #205024

   Purpose

   Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   Ancestral M.EcoGII 291
   

   Tags

   His6x tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-csgB

   Plasmid

   #89582

   Purpose

   Express csgB curli protein with GS linker and Histaq

   Depositor

   Urartu Seker

   Article

   Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

   Insert

   csgB (csgB )
   

   Tags

   3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

   Expression

   Bacterial

   Available Since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a Ov-iPGM

   Plasmid

   #180245

   Purpose

   Bacterial expression plasmid for production of recombinant Onchocerca volvulus iPGM His10

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   Onchocerca volvulus iPGM-10His
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a Wb-iPGM

   Plasmid

   #180246

   Purpose

   Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

   Depositor

   James Inglese

   Article

   van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

   Insert

   Wuchereria bancrofti iPGM-10His
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21b-P35

   Plasmid

   #11808

   Depositor

   Guy Salvesen

   Article

   Riedl et al Biochemistry. 2001 Nov 6. 40(44):13274-8

   Insert

   P35
   

   Expression

   Bacterial

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pET28-3'TwStrep

   Plasmid

   #208053

   Purpose

   Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Tags

   Twin Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-PFO

   Plasmid

   #92109

   Purpose

   Recombinant expression in E.Coli

   Depositor

   Feng Shao

   Article

   Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

   Insert

   PFO
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Delta27aa

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28 Set9 wt

   Plasmid

   #24082

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Tags

   FLAG and His

   Expression

   Bacterial

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pET28a_T5-ARG1

   Plasmid

   #106476

   Purpose

   Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

   Depositor

   Mikhail Shapiro

   Article

   Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

   Insert

   ARG1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-AlkB

    Plasmid

    #228218

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Junchao Shi

    Article

    Shi et al Nat Cell Biol. 2021 Apr;23(4):424-436. d

    Insert

    Alpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-DDX43

    Plasmid

    #134574

    Purpose

    Express human DDX43 protein (WT, full-length) in E. coli

    Depositor

    Yuliang Wu

    Article

    Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

    Insert

    DDX43 (DDX43 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    full-length WT

    Promoter

    T7

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-pchG WT

    Plasmid

    #87107

    Purpose

    expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

    Insert

    pchG WT
    

    Tags

    his-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b-irp3 WT

    Plasmid

    #87106

    Purpose

    expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

    Insert

    irp3 WT
    

    Tags

    his-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-5'TwStrep

    Plasmid

    #208620

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Tags

    Twin Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a.Tat(48-57)-EGFP

    Plasmid

    #165040

    Purpose

    Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

    Depositor

    Dokyun Na

    Article

    Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

    Insert

    EGFP
    

    Tags

    6XHis and Tat(48-57)

    Expression

    Bacterial

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-AncMEcoGII250

    Plasmid

    #205025

    Purpose

    Expressing engineered M.EcoGII with higher thermostability

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    Ancestral M.EcoGII 250
    

    Tags

    His6x tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_Caspase-4_p20_C258A

    Plasmid

    #214307

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    C258A

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-PHGDH

    Plasmid

    #74113

    Purpose

    Expression of N-terminal His-tagged

    Depositor

    Lewis Cantley

    Article

    Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    PHGDH (PHGDH Human)
    

    Tags

    His-tagged

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b_pScaC-SNAP_6xHis

    Plasmid

    #228835

    Purpose

    E. coli expression of the SNAP-tagged ScaC passenger domain

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Tags

    SNAPtag-TEV-6xHis

    Expression

    Bacterial

    Mutation

    aa 33-223

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET24c(+)-EFTu

    Plasmid

    #31172

    Depositor

    Linda Spremulli

    Article

    Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

    Insert

    E. coli elongation factor Tu (tufB E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only