We narrowed to 409 results for: RPA;

1. LENTICRISPR-UPP1_sgRNA1

   Plasmid

   #201634

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LENTICRISPR-UPP1_sgRNA2

   Plasmid

   #201635

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pWPI-UPP1-3xFLAG

   Plasmid

   #201644

   Purpose

   Over-expression of 3xFLAG-tagged human UPP1

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWPI-UPP2-3xFLAG

   Plasmid

   #201645

   Purpose

   Over-expression of 3xFLAG-tagged human UPP2

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP2 (UPP2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-TEV-U1AF37MF77M

   Plasmid

   #168267

   Purpose

   Expresses human dmU1A with the F37M/F77M double mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   U1A RRM1 crystallization module (SNRPA Human)
   

   Use

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F37M and F77M

   Promoter

   T7 promotor

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA3-TEX264-SHP1mut-cFlag

   Plasmid

   #220208

   Purpose

   Mammalian expression of TEX264 with SHP domain mutated, C-terminal Flag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   p.280GEGPL>RERPA; c.838G>C,844G>A,850T&g…

   Promoter

   CMV

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMT-EGFP-Actin5c

   Plasmid

   #15312

   Depositor

   Ron Vale

   Article

   Rogers et al J Cell Biol. 2003 Sep 15. 162(6):1079-88

   Insert

   Actin5c (Act5C Fly)
   

   Use

   Tags

   EGFP and His

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pGEX2T-TUBG1

   Plasmid

   #171967

   Purpose

   pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

   Depositor

   Maria Alvarado-Kristensson

   Article

   Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

   Insert

   TUBG1 (TUBG1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   R275H (Please see depositor comments)

   Promoter

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF1122

   Plasmid

   #143467

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HNRNPAB (HNRNPAB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TFORF1123

    Plasmid

    #143764

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HNRNPAB (HNRNPAB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. WT (DVD)

    Plasmid

    #184316

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. I282L

    Plasmid

    #184308

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I282L

    Promoter

    AOX1

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. I175L

    Plasmid

    #184322

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I175L

    Promoter

    AOX1

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. K118N

    Plasmid

    #184324

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    K118N

    Promoter

    AOX1

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. I212L

    Plasmid

    #184304

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I212L

    Promoter

    AOX1

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. I327L

    Plasmid

    #184311

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I327L

    Promoter

    AOX1

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. I309L

    Plasmid

    #184317

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I309L

    Promoter

    AOX1

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. I330L

    Plasmid

    #184320

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I330L

    Promoter

    AOX1

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. I151L

    Plasmid

    #184300

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    B-Thymosin followedby hexahistidine tag

    Expression

    Yeast

    Mutation

    I151L

    Promoter

    AOX1

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. I369L

    Plasmid

    #184318

    Purpose

    To study dynamics of G-actin using NMR spectroscopy.

    Depositor

    Michael Rosen

    Article

    Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

    Insert

    Act5C (Act5C Fly)
    

    Use

    Tags

    Hexahistidine followed by B-thymosin

    Expression

    Yeast

    Mutation

    I369L

    Promoter

    AOX1

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge