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pLenti-CMV-eGFP-hTau-WPRE-pA

Plasmid

#255025

Purpose

Lentiviral plasmid designed to express a fusion protein of enhanced GFP and human Tau

Depositor

Jonathan Ploski

Insert

Tau/MAPT (MAPT Human)

Use

Lentiviral

Available Since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Rat SARE-Arc Min Promoter-Flag Arc-SV40 3'UTR

Plasmid

#233057

Purpose

To express Flag-tagged Rat Arc from a Rat SARE-Arc minimal promoter. The Arc gene contains a SV40 3' UTR

Depositor

Jonathan Ploski

Insert

Rat Arc (Arc Rat)

Use

AAV

Tags

Flag

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pRS313-rad53-5004

Plasmid

#232902

Purpose

Plasmid carrying the rad53-5004 allele with its native promoter and terminator.

Depositor

Jolanda Van Leeuwen

Article

Batte et al Cell Syst. 2026 Mar 2:101515. doi: 10.10

Insert

RAD53 (RAD53 Budding Yeast)

Expression

Yeast

Mutation

T600C, A680G, A1094T, C1615T, C2228A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-N-3HA-GK_v1

Plasmid

#134275

Purpose

Lentivector encoding 3XHA-tagged GK (isoform 1)

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk (Gk Mouse)

Use

Lentiviral

Tags

3X HA

Expression

Mammalian

Mutation

524 amino acids, isoform 1

Promoter

CMV

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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Rnq1 (153-405)-GFP

Plasmid

#1170

Depositor

Susan Lindquist

Article

Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.

Insert

RNQ1 (153-405) (RNQ1 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pMBP-parallel1-GK5_v2

Plasmid

#134288

Purpose

Expresses GK5 (isoform 2) in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-Flag-GK5_v2

Plasmid

#134282

Purpose

Expresses Flag-tagged GK5 (isoform 2) in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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PASK gRNA (BRDN0001145672)

Plasmid

#76203

Purpose

3rd generation lentiviral gRNA plasmid targeting human PASK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PASK (PASK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHTC-HaloTag_TRIB1:1-372

Plasmid

#210969

Purpose

Mammalian expression of full-length human TRIB1. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TRIB1:M1-C372 (TRIB1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_TRIB1:1-372

Plasmid

#210968

Purpose

Mammalian expression of full-length human TRIB1. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TRIB1:M1-C372 (TRIB1 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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5'-3xFLAG Chtop-exon minimal CMV pCDNA5

Plasmid

#212080

Purpose

LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop-exon (Chtop Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Zmynd8+exon minimal CMV pCDNA5

Plasmid

#212079

Purpose

LR vector for integration of Zmynd8+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Zmynd8+exon(48nt) (Zmynd8 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 1-156

Plasmid

#140147

Purpose

GAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 1-156 (KLF4 Human)

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 157 to 479

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRDb-DN-GFP

Plasmid

#37112

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRDb-DN-GFP (ptprdb Zebrafish)

Use

Tol2 gateway destination vector

Tags

EGFP

Mutation

deleted cytodomain, including phosphatase domains…

Promoter

UAS

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

Plasmid

#89994

Purpose

Donor template for generation of NGN3-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)

Use

CRISPR

Promoter

none

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Med24+exon minimal CMV pCDNA5

Plasmid

#212061

Purpose

LR vector for integration of Med24+exon(57nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Med24+exon(57nt) (Med24 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E11

Plasmid

#154025

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 11

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-BAP1-G312G-HA

Plasmid

#154022

Purpose

Mammalian Expression of HA-tagged BAP1 with the Synonymous Mutation p.G312G

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

c.936T>G, p.G312G (Synonymous Mutation) by Sit…

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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RG6-BAP1_E7

Plasmid

#154023

Purpose

Bichromatic Fluorescent Splicing Reporter Minigene for Wild-Type BAP1 Exon 7

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)

Use

Splicing reporter

Tags

DsRed1, FLAG, SV40 NLS, and eGFP

Expression

Mammalian

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 159-329

Plasmid

#140148

Purpose

GAL4-DNA binding domain fusion protein with amino acids 159 to 329 of human KLF4 (repressor domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 159-329 (KLF4 Human)

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 1 to 158 and 330 to 479

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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