We narrowed to 1,435 results for: tk

1. pNR160

   Plasmid

   #79542

   Purpose

   Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMM763

   Plasmid

   #68900

   Purpose

   IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   NanoLuc
   

   Use

   Tags

   Expression

   Mutation

   G1104E

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAND_Int2

   Plasmid

   #60590

   Purpose

   A transcriptional AND circuit constructed by layering NOR and NOT gates

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   AND_Int2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PPhlF

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAND_yfp

   Plasmid

   #60592

   Purpose

   used with pSpec to generate an AND gate without memory

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   AND_yfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. V5-W279A-short-UBASH3A

   Plasmid

   #192086

   Purpose

   Mammalian expression of human W279A short-form UBASH3A with V5 tag

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   W279A

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNR284

   Plasmid

   #79550

   Purpose

   Output plasmid: contains 16-state register that regulates expression of GFP.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Insert

   GFPmut3b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPTK013-3a-Invertase-αMFΔ

   Plasmid

   #84972

   Purpose

   Type 3a, Invertase followed by αMFΔ - secretion signal

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Invertase followed by αMFΔ secretion tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Non

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPTK016-3-RFP_Intra

   Plasmid

   #84975

   Purpose

   Type 3, Red fluorescent protein

   Depositor

   Volker Sieber

   Article

   Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

   Insert

   Red fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   BsaI site removed (1677 c to g and 1794 a to g)

   Promoter

   Non

   Available since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JC101

   Plasmid

   #104952

   Purpose

   Methanol induing RFP, estrogen inducing GFP, and IPTG inducing CFP

   Depositor

   Timothy Lu

   Article

   Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

   Insert

   GFP, mCherry, and CFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpec

    Plasmid

    #60593

    Purpose

    used with pAND_YFP to generate an AND gate without memory

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Spec
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPTK014-3a-Killer-αMFΔ

    Plasmid

    #84973

    Purpose

    Type 3a, Killer followed by αMFΔ - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Killer followed by αMFΔ secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPTK007-3a-αMFΔ

    Plasmid

    #84966

    Purpose

    Type 3a, α-mating factor pre-sequence shortened - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    α-mating factor, pre-sequence shortened secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPTK008-3a-αMFΔ no Kex

    Plasmid

    #84967

    Purpose

    Type 3a, α-mating factor no Kex recognition site - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    α-mating factor no Kex recognition site secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPTK011-3a-SA-αMFΔ

    Plasmid

    #84970

    Purpose

    Type 3a, Serum albumin followed by αMFΔ - secretion signal

    Depositor

    Volker Sieber

    Article

    Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

    Insert

    Serum albumin followed by αMFΔ secretion tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Non

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNR186

    Plasmid

    #79552

    Purpose

    Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCis_7+8

    Plasmid

    #60587

    Purpose

    a 2-integrase plasmid

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cis_7+8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAWp7-7

    Plasmid

    #72739

    Purpose

    pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP

    Depositor

    Timothy Lu

    Article

    Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

    Insert

    UBCp-RFP-[miR-124 sensor]-CMVp-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAWp7-12

    Plasmid

    #72744

    Purpose

    pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]

    Depositor

    Timothy Lu

    Article

    Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

    Insert

    UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JC172

    Plasmid

    #104948

    Purpose

    Methanol inducing HSA-hGH fusion protein, and estrogen inducing TEV protease

    Depositor

    Timothy Lu

    Article

    Cao et al Nat Commun. 2018 Jan 8;9(1):77. doi: 10.

    Insert

    HSA-hGH fusion protein and TEV protease
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMM725

    Plasmid

    #68895

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge