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Plasmid#67159Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP6f-RS06
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationM374Q within GCaMP6fPromoterT7Available sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAV0585
Plasmid#133472PurposeIntegration into fission yeast genome at ade6 locus with hphMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVHD
Plasmid#61303PurposeVanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tagDepositorTypeEmpty backboneUseTagsHAExpressionBacterialMutationPromoterPV10Available sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pESR1-donor
Plasmid#112337PurposeCRISPR donor plasmid to tag human transcription factor ESR1 with GFPDepositorInsertESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFOXF2-donor
Plasmid#113816PurposeCRISPR donor plasmid to tag human transcription factor FOXF2 with GFPDepositorInsertFOXF2 homology arms flanking EGFP-IRES-Neo cassette (FOXF2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5
Plasmid#61257PurposeCRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2845
Plasmid#87763PurposePnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.DepositorInsertPnmr-1-PH-miniSOG
UseTagsPHExpressionWormMutationPromoterPnmr-1Available sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-cat
Plasmid#71321PurposeDonor plasmid - chloramphenicol resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNH034
Plasmid#42150DepositorInsertRT-cassette
UseTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC572
Plasmid#127973PurposeAMA1 plasmid with pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertpyrG
UseFungal expressionTagsExpressionBacterialMutationPromoternative promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3GFP
Plasmid#182205Purpose3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-TSTEP_v2
Plasmid#72336PurposeTissue-specifically swappable (C-terminal TagRFPT-to-EGFP) genome tagging plasmid with dsRed selection marker for injection mediated gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[3xP3dsRed]-RRS-EGFP
UseTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRES.Puro.EGFP
Plasmid#24176DepositorInsertEGFP
UseTagsExpressionMammalianMutationxPromoterAvailable sinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5
Plasmid#61256PurposeCRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassetteDepositorInsert2xTY1-V5
UseCRISPRTagsTY1 and V5ExpressionMutationPromoterAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM3558
Plasmid#40252DepositorInsertGm cassette (BssHII+SphI)
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc4-STRHISNDHFR
Plasmid#64000PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis & Strep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pERF-donor
Plasmid#112299PurposeCRISPR donor plasmid to tag human transcription factor ERF with GFPDepositorInsertERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOE6227.1
Plasmid#135078PurposeAn integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.DepositorInsertup- and downstream regions of upp (upp Pseudomonas putida)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAH6
Plasmid#41830PurposeUsed as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistanceDepositorTypeEmpty backboneUsePcr templateTagsExpressionBacterial and YeastMutationPromoterT7,TEFAvailable sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRVV661
Plasmid#108282PurposeDonor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9DepositorInsertPhiC31 attP sites
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pISL1-donor
Plasmid#113820PurposeCRISPR donor plasmid to tag human transcription factor ISL1 with GFPDepositorInsertISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)
UseCRISPRTagsExpressionMutationUnknownPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-hph
Plasmid#71322PurposeDonor plasmid - hygromycin resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-cHAIAA17
Plasmid#48990Purposefor Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsIAA17 degron tagExpressionMutationPromoterAvailable sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBS-MCS1M(8)Kana-mCMV-pA
Plasmid#22059DepositorInsertmCMV-pA expression cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_5a6.10
Plasmid#178934PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
CFP-CitA6-130-Venus
Plasmid#128016PurposeUnmodified FLIP CitA6-130 sensorDepositorInsertCitA
UseTagsExpressionMutationsee Depositor CommentsPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC573
Plasmid#127974PurposeAMA1 plasmid with argB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertargB
UseFungal expressionTagsExpressionBacterialMutationPromoternative promoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKB
Plasmid#12499DepositorInsertFKB
UseTags6xHisExpressionBacterialMutationRosettaDesign was used to design sequences. Align…PromoterAvailable sinceMarch 13, 2007AvailabilityAcademic Institutions and Nonprofits only -
1xTRE DREaMR 3p3GFP
Plasmid#182203Purpose1xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-spo5DSR
Plasmid#48983Purposefor Cre-lox cassette exchange of untagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only