We narrowed to 30,517 results for: ide

1. pGSKU

   Plasmid

   #72243

   Purpose

   Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

   Depositor

   Francesca Storici, Michael Resnick

   Article

   Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

   Inserts

   KlURA3
   kanMX4
   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC1-HyPer-Red-C199S

   Plasmid

   #48252

   Purpose

   Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HYPER-RED-C199S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1m-ELP

   Plasmid

   #170763

   Purpose

   EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

   Depositor

   Andrew MacKay

   Article

   Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

   Insert

   EF1m-ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDECKO_mCherry

   Plasmid

   #78534

   Purpose

   Backbone to clone single or paired sgRNAs

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (for expressing sgRNA)

   Available since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC1-HyPerRed-mito

   Plasmid

   #60247

   Purpose

   Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HyPerRed-mito
   

   Use

   Tags

   Mitochondrial targeting signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. modified pcAT7-Glo1

   Plasmid

   #160996

   Purpose

   Splicing reporter backbone

   Depositor

   Brenton Graveley

   Article

   Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

   Insert

   hemoglobin subunit beta (HBB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Repeated intron 1 with 40 nucelotide deletion

   Promoter

   CAG

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIX403-hNOS2

   Plasmid

   #110800

   Purpose

   tet-inducible NOS2 expression

   Depositor

   Edward Morgan

   Article

   Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

   Insert

   NOS2 (NOS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

   Plasmid

   #228721

   Purpose

   Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGSHU

   Plasmid

   #72244

   Purpose

   Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

   Depositor

   Francesca Storici, Michael Resnick

   Article

   Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

   Inserts

   KlURA3
   hyg
   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmCherry_gRNA

    Plasmid

    #80457

    Purpose

    gRNA expression vector with mCherry transfection control

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Inserts

    sp Cas9 gRNA
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin promoter

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPS22 (pET23a-Cas10/Csm/csm2H6N)

    Plasmid

    #70038

    Purpose

    Bacterial expression of the S. epidermidis Cas10·Csm complex

    Depositor

    Luciano Marraffini

    Article

    Hatoum-Aslan et al J Biol Chem. 2013 Sep 27;288(39):27888-9

    Insert

    Cas10/Csm complex
    

    Use

    Tags

    6xHIS on N terminus of Csm2

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. modRNAc0-p53DD

    Plasmid

    #176902

    Purpose

    IVT template plasmid for making p53DD modRNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    p53DD
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS2191_Nme2-evoFERNY-nt

    Plasmid

    #201527

    Purpose

    Mammalian expression of N-terminally fused Nme2-evoFERNY-nt

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-evoFERNY-nt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPIDNB2

    Plasmid

    #169902

    Purpose

    Empty dox inducible vector with piggyBac, insulators, and a DTS. The constitutive mNeonGreen is fused to H2B to allow for visualization of the nucleus.

    Depositor

    Richard Schneider

    Article

    Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

    Type

    Empty backbone

    Use

    Avian expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dox inducible

    Available since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSR0

    Plasmid

    #161984

    Purpose

    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

    Depositor

    Olga Soutourina

    Article

    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNTI661 pRPR1(TetO)-sgRNA

    Plasmid

    #139475

    Purpose

    Vector for tet-regulated sgRNA expression in yeast

    Depositor

    Nicholas Ingolia

    Article

    McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

    Insert

    single guide RNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pRPR1(TetO)

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. ratGlp1-R

    Plasmid

    #14944

    Depositor

    Bernard Thorens

    Article

    Thorens et al Proc Natl Acad Sci U S A. 1992 Sep 15. 8

    Insert

    Glp1-R (Glp1r Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pEJS2195_Nme2-evoFERNY-i1

    Plasmid

    #201528

    Purpose

    Mammalian expression of domain inlaid Nme2-evoFERNY-i1

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-evoFERNY-i1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS2197_Nme2-evoFERNY-i8

    Plasmid

    #201530

    Purpose

    Mammalian expression of domain inlaid Nme2-evoFERNY-i8

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-evoFERNY-i8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge