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pGL0_16 [2μ ORI]

Plasmid

#198929

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

2μ ORI

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE myc-Raptor

Plasmid

#18116

Depositor

Reuben Shaw

Article

Gwinn et al Mol Cell. 2008 Apr 25. 30(2):214-26.

Insert

Raptor (RPTOR Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pAL-rfp_T

Plasmid

#74075

Purpose

pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

sgRNA rfp (T)

Use

Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSG5-BBLF2/3

Plasmid

#72633

Purpose

Mammalian expression vector for Epstein-Barr virus primase associated factor

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BBLF2/3

Expression

Mammalian

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pRW398-URA3-BXB1-LP

Plasmid

#235977

Purpose

Plasmids for generation of donor DNA for construction of a BXB1-compatible landing pad p1 with URA3 selection marker.

Depositor

Chang Liu

Article

Pisera et al ACS Synth Biol. 2025 Apr 18;14(4):1002-1

Insert

URA3

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RR-zrif1_exon8

Plasmid

#194434

Purpose

Right (RR) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_RIGHT (rif1 Zebrafish)

Use

TALEN

Promoter

CMV IE94

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-DD-zrif1_exon8

Plasmid

#194435

Purpose

Left (DD) TALEN for mutating zebrafish rif1 gene

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

zrif1_ex8_LEFT (rif1 Zebrafish)

Use

TALEN

Promoter

CMV IE94

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pRSII42N-TDH3pr

Plasmid

#194531

Purpose

High copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

Depositor

Patrick Gibney

Article

Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pOPING

Plasmid

#26046

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

SS peptide-LacZ-KHHHHHH

Tags

KHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETG

Expression

Bacterial, Insect, and Mamm…

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pETDuet-T7-Ct-YhjH

Plasmid

#79167

Purpose

Dual expression of Ct biosensor and YhjH under individual T7 promoters

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Inserts

yhjH
T7-Spinach2-Ct

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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T10-T7-hMOV10

Plasmid

#185052

Purpose

expression of WT human MOV10

Depositor

Constance Ciaudo

Article

Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

Insert

cDNA WT hMOV10 (MOV10 Human)

Tags

T7

Expression

Mammalian

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBSV2G_2

Plasmid

#118225

Purpose

Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGL0_29 [aph(3')-I EZ-Tn5]

Plasmid

#198942

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

aph(3')-I EZ-Tn5

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pFSy(1.1)GW

Plasmid

#27232

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pXP418

Plasmid

#26843

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pT7tyrgRNA

Plasmid

#46761

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

tyr gRNA (tyr Zebrafish)

Use

CRISPR

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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SAP130

Plasmid

#11241

Depositor

Robin Reed

Article

Das et al Mol Cell Biol. 1999 Oct . 19(10):6796-80

Insert

SAP130 (SF3B3 Human)

Expression

Bacterial

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Expression

Bacterial and Yeast

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDR-GW FLIPglu-600μΔ13V

Plasmid

#28001

Depositor

Wolf Frommer

Article

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

FLIPglu-600μΔ13V

Tags

eCFP and eYFP

Expression

Yeast

Mutation

F16A in mglB

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
6798 pJC25NMstop

Plasmid

#1228

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

NM (SUP35 Budding Yeast)

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only