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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,206 results for: sgRNA

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Showing: 3781 - 3800 of 9206 results

  1. B-evoSpCas9

    Plasmid
    #126765
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126765-plasmid-map-sequence-id-410921

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  2. B-HeFSpCas9

    Plasmid
    #126766
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HeFSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126766-plasmid-map-sequence-id-259607

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  5. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-487610

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  8. pXPR_045

    Plasmid
    #107144
    Purpose
    cloning of a sgRNA
    Depositor
    David Root
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107144-plasmid-map-sequence-id-205294

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  11. pKLV2-U6gRNA5(sgBart8-5p)-PGKpuro2ABFP-W

    Plasmid
    #252919
    Purpose
    Expression vector for gRNA with Puro and tagBFP selection markers
    Depositor
    Sok Ching Cheong
    Insert
    EBV-miR-Bart8-5p
    Use
    CRISPR
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    252919-plasmid-map-sequence-id-508266

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  12. pKLV2-U6gRNA5(sgBart11-5p)-PGKpuro2ABFP-W

    Plasmid
    #252921
    Purpose
    Expression vector for gRNA with Puro and tagBFP selection markers
    Depositor
    Sok Ching Cheong
    Insert
    EBV-miR-Bart11-5p
    Use
    CRISPR
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    252921-plasmid-map-sequence-id-507824

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  16. pKLV2-U6gRNA5(sgBart10-5p)-PGKpuro2ABFP-W

    Plasmid
    #252920
    Purpose
    Expression vector for gRNA with Puro and tagBFP selection markers
    Depositor
    Sok Ching Cheong
    Insert
    EBV-miR-Bart10-5p
    Use
    CRISPR
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    252920-plasmid-map-sequence-id-505089

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Showing: 3781 - 3800 of 9206 results