We narrowed to 5,822 results for: pcas

1. pEF1a-PEmax-P2A-GFP

   Plasmid

   #202000

   Purpose

   Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

   Depositor

   Daniel Geschwind

   Insert

   PEmax-P2A-GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-ABE-P48R

   Plasmid

   #161816

   Purpose

   Expresses ABE-P48R in mammalian cells

   Depositor

   Sangsu Bae

   Insert

   bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)

   Plasmid

   #160607

   Purpose

   TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.

   Depositor

   Diego Orzaez

   Insert

   CUP2Gal4AD
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. ptubP-QS

   Plasmid

   #24352

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   QS
   

   Tags

   white gene

   Expression

   Insect

   Available Since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRho-Cre

   Plasmid

   #13779

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Rhodopsin promoter-Cre
   

   Use

   Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. miR-TDP43b

   Plasmid

   #28212

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   miRNAb
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNdrg4-DsRed

   Plasmid

   #13766

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Ndrg4 promoter
   

   Tags

   DsRed2

   Expression

   Mammalian

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

   Plasmid

   #160618

   Purpose

   Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

   Depositor

   Diego Orzaez

   Insert

   Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos, 35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. miR-TDP43a

   Plasmid

   #28211

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   miRNAa
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. VIPR2

    Plasmid

    #39044

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    VIPR2 (VIPR2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

11. HiFi dFE

    Plasmid

    #190142

    Purpose

    Expresses HiFiCas9-T4pol (HiFi dFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    HiFi Cas9-T4pol (HiFi dFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    SpCas9 R691A mutant, Codon Optimized for mammalia…

    Promoter

    CBh

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNSU299_RPL39

    Plasmid

    #166078

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RPL39 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of RPL39 (Overlaps with 5'UTR and first base of gene) (RPL39 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    Tet-inducible

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNSU299_YDR344C

    Plasmid

    #166072

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA targeting intergenic site near HXT6 (within YDR344C) for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Intergenic site near HXT6 (in YDR344C, possible dubious ORF)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    Tet-inducible

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNSU299_TDH3

    Plasmid

    #166084

    Purpose

    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of TDH3 for double stranded break formation in yeast.

    Depositor

    Rebecca Burgess

    Insert

    Promoter of TDH3 (TDH3 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    Tet-inducible

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)

    Plasmid

    #160631

    Purpose

    Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.

    Depositor

    Diego Orzaez

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U626

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)

    Plasmid

    #160640

    Purpose

    Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

    Depositor

    Diego Orzaez

    Insert

    Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos, 35S

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CJ30 (miR-1003 minigene)

    Plasmid

    #17767

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    dme-miR-1003 minigene
    

    Use

    Drosophila p-element vector

    Available Since

    May 12, 2008

    Availability

    Academic Institutions and Nonprofits only

18. PBCAG-eGFP

    Plasmid

    #40973

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Inserts

    PiggyBac transposon 3' terminal repeats
    PiggyBac 5' terminal repeats
    

    Expression

    Mammalian

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330.puro

    Plasmid

    #110403

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCX138

    Plasmid

    #229213

    Purpose

    CRISPR array plasmid for NOTCH2 (Spacers 25-48)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for NOTCH2 (Spacers 25-48)
    

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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