We narrowed to 4,436 results for: Abo

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pX459_gRNA-ROSA26_hspCas9

Plasmid

#193310

Purpose

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pMD2.G-H8A/K47Q

Plasmid

#207322

Purpose

VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSV-G (VSIVgp4 Vesicular Stomatitis Virus)

Expression

Mammalian

Mutation

H8A/K47Q

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD18HMGR

Plasmid

#19041

Depositor

Jay Keasling

Article

Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

Insert

truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pB6-Hipp11-H2B-sfGFP-T2A-tdTomato-cre reporter-3pA stop-FNF-DTA

Plasmid

#183027

Purpose

B6J Hipp11-FNF-pCAG-loxP-3xSV40pA-loxP-H2B-sfGFP-T2A-tdTomato-WPRE-bpA allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

H2B-sfGFP-T2A-tdTomato

Use

Cre/Lox and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pB6-Gfap-T2A-tdTomato-FNF-DTA

Plasmid

#140271

Purpose

B6J Gfap-T2A-tdTomato allele targeting vector, low copy number

Depositor

Mario Capecchi

Insert

Gfap (Gfap Mouse)

Use

Mouse Targeting

Tags

C-terminal fusion of T2A-tdTomato

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Csy4H29AS50C

Plasmid

#45029

Depositor

Jennifer Doudna

Article

Lee et al Proc Natl Acad Sci U S A. 2013 Apr 2;110

Insert

Csy4H29AS50C

Tags

His6-MBP

Expression

Bacterial

Mutation

H29A mutation to abolish catalytic activity and S…

Promoter

T7

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pRK5-SNAP-CLIP-GB1a

Plasmid

#125709

Purpose

Eukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptor

Depositor

Kai Johnsson

Article

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Insert

SNAP-CLIP-GB1a

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-loxP-3xpA-loxP-sfGFP

Plasmid

#166126

Purpose

sfGFP cre reporter

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

sfGFP

Use

Cre/Lox

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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JAB2240

Plasmid

#190359

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::PhlF-NLS-19Sterm)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::PhlF-NLS-19Sterm

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRS425ADS

Plasmid

#20119

Depositor

Jay Keasling

Article

Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

Insert

amorphadiene synthase

Use

Synthetic Biology

Expression

Yeast

Available Since

March 2, 2009

Availability

Academic Institutions and Nonprofits only
pAEF5

Plasmid

#136305

Purpose

Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss

Depositor

Gilles Fischer

Article

Agier et al Methods Mol Biol. 2021;2196:181-198. doi

Type

Empty backbone

Use

CRISPR

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pKS101

Plasmid

#24631

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

FAA2 (FAA2 Budding Yeast)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
pBbA5k-EPL14

Plasmid

#45405

Depositor

Aindrila Mukhopadhyay

Article

Dunlop et al Mol Syst Biol. 2011 May 10;7:487. doi: 1

Insert

mexF, PP_3426

Use

Synthetic Biology

Tags

His

Promoter

LacUV5

Available Since

Jan. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pQP-2473

Plasmid

#138976

Purpose

Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-P.accABCD

Plasmid

#66866

Purpose

Encodes accA, accB, accC, accD in Pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

accA
accB
accC
accD

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pMTG07-Amp-FRT

Plasmid

#128276

Purpose

Gene circuit with the following architecture: 5xUAS-mNeonGreen--CMV-Gal4-VVD-p65

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Insert

Gal4-VVD-p65

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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JAB2282

Plasmid

#190363

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-ERF4(RD)-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-ERF4(RD)-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2497

Plasmid

#190378

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::QacR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::QacR-NLS_19St

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2657

Plasmid

#190393

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::LacI-NLS-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::LacI-NLS-19St

Expression

Plant

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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