We narrowed to 8,678 results for: Pet2;

1. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

   Plasmid

   #229223

   Purpose

   For expression of T. elongatus KaiB protein mutant

   Depositor

   Andy LiWang

   Insert

   kaiB (kaiB T. elongatus)
   

   Tags

   FLAG and SUMO-FLAG

   Expression

   Bacterial

   Mutation

   1-94 (truncated), Y8A, Y94A

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

   Plasmid

   #229223

   Purpose

   For expression of T. elongatus KaiB protein mutant

   Depositor

   Andy LiWang

   Insert

   kaiB (kaiB T. elongatus)
   

   Tags

   FLAG and SUMO-FLAG

   Expression

   Bacterial

   Mutation

   1-94 (truncated), Y8A, Y94A

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

   Plasmid

   #226349

   Purpose

   Expression of Titin V15P substrate with GS tail

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

   Plasmid

   #226349

   Purpose

   Expression of Titin V15P substrate with GS tail

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

   Plasmid

   #169135

   Purpose

   E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Coh7
   

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

   Plasmid

   #169135

   Purpose

   E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Coh7
   

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

   Plasmid

   #208626

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
   

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 191~307, other residues not included

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

   Plasmid

   #208627

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
   

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 299~398, other residues not included

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

   Plasmid

   #208629

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
   

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 212~285, other residues not included

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid

    #208626

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 191~307, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid

    #208627

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 299~398, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid

    #208629

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 212~285, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176575

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176575

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175788

    Purpose

    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175788

    Purpose

    Plasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

    Plasmid

    #175801

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis

    Plasmid

    #175801

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Tags

    AviTag, Hexahistidine tag, and eGFP

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

    Plasmid

    #157672

    Purpose

    Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
    

    Tags

    ddFLN4, ELP, Histag and ybbR tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR

    Plasmid

    #157672

    Purpose

    Expression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
    

    Tags

    ddFLN4, ELP, Histag and ybbR tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge