We narrowed to 7,489 results for: addgene/1000

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pGL4.24-Cd36-enhancer8

Plasmid

#138580

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17923535_17924934

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-CDC42

Plasmid

#109583

Purpose

Multisite gateway vector for 3' tagging with CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-deltaA1-Lock-VWF

Plasmid

#124824

Purpose

Expresses multimeric human VWF protein without A1 domain and Lock A2 domain. This protein has C-terminal c-Myc tag.

Depositor

Sriram Neelamegham

Article

Zhang et al Blood Adv. 2019 Apr 9;3(7):957-968. doi:

Insert

VWF without A1 domain and Lock A2 domain (VWF Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmNanoLuc

Plasmid

#182969

Purpose

Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmNanoLuc

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_KCNA1-mcherry

Plasmid

#159788

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human KCNA1 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting KCNA1 gene (KCNA1 Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v2 puro

Plasmid

#87793

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP5.v1 puro

Plasmid

#87792

Purpose

Expresses an inducible short hairpin targeting human MKP5 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP5 (DUSP10 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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PA-mCherry1-ER-5

Plasmid

#57174

Purpose

Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

York et al Nat Methods. 2011 Apr;8(4):327-33. doi:

Insert

ER (CALR Human)

Tags

PA-mCherry1

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only
pBabe EGFR Del4(T790M)

Plasmid

#32074

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete L747-P753, insert S, T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pX459-HypaCas9-mR2-TUBA1B_sgRNA

Plasmid

#183888

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

TUBA1B sgRNA spacer (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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DSCAML1-CD4d3+4-bio

Plasmid

#73105

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding DSCAML1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

DSCAML1 (DSCAML1 Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pF714-pET28a-Hs-FL-METTL20-NHis wt

Plasmid

#85115

Purpose

expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length METTL20 (METTL20 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only
LDN

Plasmid

#21195

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek1 (Esr1, MAP2K1 Human, Mouse)

Use

Retroviral

Mutation

Mek1 K97M fused to ER*, catalytically inactive

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
LXSN-Cx43

Plasmid

#60692

Purpose

retroviral expression of rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
mCerulean3-Integrin-Beta1-N-18

Plasmid

#55423

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-73) (72P)

Plasmid

#62083

Purpose

Expression of human p53 (1-73) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72P

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pGL4.24-Cd36-enhancer7

Plasmid

#138579

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17918617_17920400

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.24-Cd36-enhancer6

Plasmid

#138578

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17898458_17899478

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.24-Cd36-enhancer3

Plasmid

#138575

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17848003_17849903

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.24-Cd36-enhancer2

Plasmid

#138574

Purpose

Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17843052_17844983

Depositor

Lora Hooper

Article

Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

Insert

Cd36 Promoter (Cd36 Mouse)

Use

Luciferase

Promoter

Minimal promoter

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge