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pCG-A-celA(SP)-B

Plasmid

#87371

Purpose

An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

CelA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-xynA(SP)-B

Plasmid

#87372

Purpose

An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

XynA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAJ09

Plasmid

#14913

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis G protein alpha subunit promoter

Tags

GFP(S65C)

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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knob-s48i48

Plasmid

#68391

Purpose

Knob protein fused to elastin-like polypeptide (S48I48)

Depositor

Andrew MacKay

Article

Sun et al J Control Release. 2011 Oct 30;155(2):21

Insert

Knob-S48I48

Tags

Knob

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-A

Plasmid

#59792

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele A

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele A

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pPI457

Plasmid

#113200

Purpose

shuttle vector, C-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, c-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPI418

Plasmid

#113199

Purpose

shuttle vector, N-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, n-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMM725

Plasmid

#68895

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMM750

Plasmid

#68899

Purpose

IPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pDM1019

Plasmid

#108976

Purpose

shuttle vector, N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector

Tags

GFP

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET26-CNTN149S/F366A

Plasmid

#100165

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Mutation

N149S, F366A

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDM1220

Plasmid

#108993

Purpose

integrating expression vector

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum integrating expression v…

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMM732

Plasmid

#68897

Purpose

IPTG-inducible CRISPRi vector targeting NanoLuc (NL2), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pUASTattb AaegOr4-C

Plasmid

#59794

Purpose

UAS-induced expression of Aedes aegypti odorant receptor 4 (OR4) cDNA allele C

Depositor

Leslie Vosshall

Article

McBride et al Nature. 2014 Nov 13;515(7526):222-7. doi

Insert

Aedes aegypti Or4 cDNA allele C

Expression

Insect

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAJ02

Plasmid

#14909

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pET21a.TM1363

Plasmid

#11420

Depositor

Sung-Hou Kim

Article

Shin et al J Mol Biol. 2004 Jul 30. 341(1):227-39.

Insert

peptide release factor I (prfA Thermotoga maritima)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pAM-CaMKII-EGFP-T2A-FlipO

Plasmid

#232791

Purpose

AAV2 vector containing EGFP and FlipO sequences separated by T2A peptide under the control of CaMKIIA promoter.

Depositor

Peer Wulff

Article

Baccini et al bioRxiv 2024.08.13.606964

Insert

CaMKII-EGFP-T2A-FlipO

Use

AAV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-FLEX-hM4D(Gi)-mCherry

Plasmid

#232782

Purpose

AAV2 vector containing Gi-coupled hM4D DREADD fused with mCherry inside a double floxed (FLEX) cassette under the control of CBA promoter.

Depositor

Peer Wulff

Article

Baccini et al bioRxiv 2024.08.13.606964

Insert

hM4D(Gi)-mCherry

Use

AAV

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-TO-g0 (FLP-IN)

Plasmid

#236120

Purpose

Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

g0

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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