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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,508 results for: nls

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Showing: 3841 - 3860 of 6508 results

  3. Sc++

    Plasmid
    #198545
    Purpose
    Expresses human codon-optimized Sc++ and blasticidin resistance: EFS promoter-Sc++-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    Sc++
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198545-plasmid-map-sequence-id-388902

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  4. SpCas9-NRRH

    Plasmid
    #198540
    Purpose
    Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRRH
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198540-plasmid-map-sequence-id-388897

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  5. GB3668

    Plasmid
    #215243
    Purpose
    TU for the expression of mAID (N-tag): AcrIIA4 w/o NLS protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and TNOS terminator
    Depositor
    Diego Orzaez
    Article
    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47
    Insert
    P35S:mAID:AcrIIA4:TNOS
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    215243-plasmid-map-sequence-id-436560

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  11. pEG302-SunTagng-22aa

    Plasmid
    #115345
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome
    Depositor
    Steven Jacobsen
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    115345-plasmid-map-sequence-id-222916

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  12. pStr-KDEL-IRES-ST-mCherry

    Plasmid
    #110727
    Purpose
    Expresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherry
    Depositor
    David Stephens
    Article
    McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180
    Inserts
    Str-KDEL
    STase sialyltransferase (ST6GAL1 Human)
    Tags
    Str Streptavidin binding peptide (SBP) and mCherr…
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 43 of ST
    Promoter
    CMV and IRES
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    110727-plasmid-map-sequence-id-259745

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  15. pLenti EF1a mCherry-T2A-VP64-NtcA-2xNLS_SV40

    Plasmid
    #254310
    Purpose
    Constitutive expression of VP64-NtcA-2xNLS_SV40 for use in the functional aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid. mCherry to monitor NtcA chimera expression.
    Depositor
    Samuel McBrayer
    Article
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and FLAG-mCherry-T2A
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254310-plasmid-map-sequence-id-508529

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  16. pLenti EF1a mCherry-T2A-VP64-NtcA_R90E-2xNLS_SV40

    Plasmid
    #254311
    Purpose
    Constitutive expression of VP64- NtcA_R90E-2xNLS_SV40 for use in the inactive aKG-ON biosensor. Requires pLenti Neo glnA PCC 6803 reporter gene plasmid. mCherry to monitor NtcA fusion expression.
    Depositor
    Samuel McBrayer
    Article
    Sternisha et al Science. 2026 Jul 16;393(6808):eadx8675.
    Insert
    VP64-NtcA_R90E-2xNLS_SV40
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and FLAG-mCherry-T2A
    Expression
    Mammalian
    Mutation
    NtcA R90E
    Promoter
    EF1a
    Available Since
    July 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    254311-plasmid-map-sequence-id-508538

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  19. pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

    Plasmid
    #226114
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226114-plasmid-map-sequence-id-448323

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  20. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

    Plasmid
    #211844
    Purpose
    Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.
    Depositor
    Marcela Rojas-Pierce
    Article
    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.
    Insert
    LRR-NEL
    Use
    Synthetic Biology
    Tags
    3xNLS-HA and mCherry
    Expression
    Plant
    Mutation
    C492A
    Promoter
    UBIQUITIN10
    Available Since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    211844-plasmid-map-sequence-id-416933

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Showing: 3841 - 3860 of 6508 results