We narrowed to 5,323 results for: pcDNA3.1

1. pcDNA3.1 2xFLAG-2xSTREP-Emsy (mouse)

   Plasmid

   #236419

   Purpose

   transient overexpression of EMSY in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   EMSY (2210018M11Rik Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 2xFLAG-2xSTREP-EMSY (human)

   Plasmid

   #236420

   Purpose

   transient overexpression of EMSY in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   EMSY (EMSY Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

   Plasmid

   #236422

   Purpose

   transient overexpression of KEAP1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   KEAP1 (KEAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

   Plasmid

   #238007

   Purpose

   300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

   Depositor

   Agnel Sfeir, Simon Powell

   Article

   Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

   Insert

   AAVS1 (AAVS1, PPP1R12C Human)
   

   Use

   Rna donor to study rt-dsbr

   Tags

   Expression

   Mammalian

   Mutation

   3bp insertion signature (ATC) at Cas9 cut site in…

   Promoter

   CMV

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

   Plasmid

   #233064

   Purpose

   Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

   Plasmid

   #233063

   Purpose

   Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Threonine 923 to Glycine

   Promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 Cas9-P2A-dTomato-PS9

   Plasmid

   #231911

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 Cas9-P2A-dTomato-T20

   Plasmid

   #231912

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 T21R-Nb139-T2A-mCherry

   Plasmid

   #225589

   Purpose

   Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-Nb139-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

    Plasmid

    #228532

    Purpose

    A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Skp1(dC111):gs10:FRB:HA (SKP1 Human, Synthetic)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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