We narrowed to 10,332 results for: transfer
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Plasmid#213684PurposeExpresses human granulin-4 with an N-terminal twin-Strep-FLAG tagDepositorInsertHuman granulin-4 (hGRN4) (GRN Human)
UseAAVTagsTwin-Strep tag and FLAG tag (after signal peptide…Promotercytomegalovirus enhancer/chicken β-actin promoterAvailable SinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry
Plasmid#208835PurposeKnockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoterDepositorInsertmCherry
UseAAV and CRISPRExpressionBacterial and MammalianPromoterhSyn1Available SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry
Plasmid#208836PurposeKnockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoterDepositorInsertmCherry
UseAAV and CRISPRExpressionBacterial and MammalianPromoterhSyn1Available SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry
Plasmid#208834PurposeControl plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFPDepositorInsertmCherry
UseAAV and CRISPRExpressionBacterial and MammalianPromoterhSyn1Available SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-scFVDnmt3A_bGHpA
Plasmid#177349PurposeAAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsmyc and scFVExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterCMVAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVDnmt3A_bGHpA
Plasmid#177355PurposeAAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsmyc and scFVExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterTetracycline-dependent promoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVDnmt3L_bGHpA
Plasmid#177356PurposeAAV expression of scFV-fused C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertC-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
UseAAVTagsmyc and scFVExpressionMammalianMutation194–415 aaPromoterCMV promoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVtet1_bGHpA
Plasmid#177357PurposeAAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationN terminal domain of human Tet1PromoterTetracycline-dependent promoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1297-AAV-EFSNC-dCjCas9-HP1b(79-173)
Plasmid#223142PurposeExpression of truncated HP1b with dCjCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 79-173PromoterEF1aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)
Plasmid#223162PurposeExpression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1298-AAV-EFSNC-dCjCas9-HP1bNH(111-173)
Plasmid#223143PurposeExpression of truncated HP1b with dCjCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 111-173PromoterEF1aAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tnnt2-Cre-U6-Lmna-sgRNA
Plasmid#206178PurposeExpresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10DepositorInsertCre
UseAAVPromotercTnTAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1308-AAV-EFSNC-dSaCas9-HP1bNH(111-173)
Plasmid#223153PurposeExpression of truncated HP1b with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 111-173PromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1307-AAV-EFSNC-dSaCas9-HP1b(79-173)
Plasmid#223152PurposeExpression of truncated HP1b with dSaCas9 and empty gRNA scaffoldDepositorInsertHP1b (CBX1 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 79-173PromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1861-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)
Plasmid#223161PurposeExpression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)
Plasmid#223166Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti HsATP13A2 P652_E653 del
Plasmid#213700Purposetransfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 delDepositorAvailable SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCHpr-DREADD (Gq)-mCherry
Plasmid#221036PurposeExpresses Gq coupled DREADD hM3D under the rat pro-melanin-concentrating hormone promoterDepositorInsertDREADD Gq-mCherry
UseAAV; Rat targetingExpressionMammalianPromoterRat MCHAvailable SinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cTNT-GFP-Palmd
Plasmid#216832Purposecardiomyocytes-specific plasmid, expressing Gfp and the coding sequence of mouse PalmdDepositorAvailable SinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-cTNT-NEXN-GFP
Plasmid#216835Purposecardiomyocytes-specific plasmid, expressing the coding sequence of mouse Nexn and GFP tagDepositorAvailable SinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTFG002-ACE2(Mut)-HA
Plasmid#191570PurposeACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(Mut)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA Tag.DepositorInsertAngiotensin Converting Enzyme II (ACE2 Human)
UseLentiviral and RNAiTagsHA tagExpressionMammalianMutationCodon-optimized for human expression. Also contai…PromoterCMVAvailable SinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTFG001-ACE2(WT)-HA
Plasmid#191569PurposeACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.DepositorInsertAngiotensin Converting Enzyme II (ACE2 Human)
UseLentiviral and RNAiTagsHA tagExpressionMammalianMutationCodon optimized for expression in human cellsPromoterCMVAvailable SinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-OFF-ArgiNLS-AausFP1
Plasmid#220605PurposeFlp-independent expression of a single-cell discriminating version of AausFP1 fluorescent protein. Flp activity can turn off expression.DepositorInsertAausFP1
UseAAVTagsArgiNLSExpressionMammalianPromoterCAGAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-UDP1.0-WPRE
Plasmid#220849PurposeAAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)DepositorInsertUDP1.0
UseAAVPromoterhuman Synapsin (hSyn)Available SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-flex-iGABASnFR2n-WPRE
Plasmid#218881PurposeAAV-mediated expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
UseAAV and Cre/LoxExpressionMammalianMutationS99A F104H R168PPromoterCAGAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE
Plasmid#218873PurposeAAV-mediated expression of improved GABA sensor control (no change in fluorescence)DepositorHas ServiceAAV1 and AAV5InsertiGABASnFR2(no bind)
UseAAVExpressionMammalianMutationS99A F102G R168PPromoterGFAPAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN
Plasmid#209786PurposeExpresses TadA8e and SpRY cas9N by the specific TNT4 promoterDepositorInsertTNT4, TadA8e, SpRY N, inteinN
UseAAVPromoterTNT4Available SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_FKBP-V5-PRRX1
Plasmid#215024PurposeAAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for human PRRX1DepositorAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-6xTRE-FLEX-hM4Di.mCherry
Plasmid#169415PurposeActivity-dependent, cre-dependent, temporally restricted inhibitory DREADD expressionDepositorInserthM4Di.mCherry
UseAAVTagsmCherryMutationhM4Di is Gi-coupled DREADD.Derived from human mus…PromoterMinimal TATAAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W
Plasmid#200502PurposeLentiviral vector expressing gRNA targeting human AAVS1DepositorInsertAAVS1 (AAVS1 Human)
UseLentiviralAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only