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pET28A-p53 (1-73) (P to A)

Plasmid

#62069

Purpose

Expression of human p53 (1-73) (all P to A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; all Prolines changed …

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
scaffold-linker-U6

Plasmid

#89639

Purpose

construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304-MARK3_T211A

Plasmid

#107236

Purpose

Lentiviral gene expression vector for phospho-MARK3_T221A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRecLV105

Plasmid

#107238

Purpose

A lentiviral vector for expression in mammalian cells

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pINDUCER20 MEF2C S222A

Plasmid

#89718

Purpose

Lentiviral gene expression vector for doxycycline inducible MEF2C-S222A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed serine 222 to alanine

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFPLaminA-N195K

Plasmid

#32708

Depositor

Wendy Bickmore

Article

Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

Insert

Lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Mutation

N195K

Promoter

CMV

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MMP8 (P78S)

Plasmid

#29546

Depositor

Yardena Samuels

Article

Palavalli et al Nat Genet. 2009 May . 41(5):518-20.

Insert

Metalloproteinase protein 8 (MMP8 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

P78S

Available Since

July 22, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-HsRCK_E

Plasmid

#146210

Purpose

Bacterial Expression of HsRck

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsRck (DDX6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pNC-Cam3304-RNAi

Plasmid

#193401

Purpose

Nimble cloning-compatible vector for plant RNAi

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBXNH3LCA_TM287/288

Plasmid

#132701

Purpose

expresses TM287/288 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

TM287/288 (TM0288, TM0287 Thermotoga maritima)

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFSy(336)GW

Plasmid

#27231

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pSALECT-Efnb2

Plasmid

#59707

Purpose

pSALECT with Efnb2 insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

Efnb2 (Efnb2 Mouse)

Expression

Bacterial

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pSALECT-Ham1

Plasmid

#59706

Purpose

pSALECT with Ham1 insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

Ham1

Expression

Bacterial

Available Since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pRetroSuper TIF1gamma

Plasmid

#15728

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma shRNA (TRIM33 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pGEX Smad2 (L+MH2)

Plasmid

#15730

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad2 (L+MH2) (SMAD2 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 187-467 (linker and MH2 regions).

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pPIPL12-FnCas12a

Plasmid

#199563

Purpose

Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

Fncas12a

Use

CRISPR and Synthetic Biology; Tetracycline induci…

Expression

Bacterial

Promoter

COtetR-PIPL12

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-M-Flag-BAP1-C91G

Plasmid

#125845

Purpose

express mutant BAP1-C91G

Depositor

Boyi Gan

Article

Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

Insert

BAP1 (BRCA1 associated protein 1) (BAP1 Human)

Use

Lentiviral

Tags

Flag

Mutation

C91G

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

Plasmid

#225242

Purpose

Mammalian expression of iPASnFR-L in the cytosol

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pZE-PpadC-yveF-yveG-egfp

Plasmid

#201791

Purpose

the promoter can be recognized by PadR

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PpadC-yveF-yveG

Expression

Bacterial

Mutation

WT

Available Since

July 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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CIp10 - ffDronpa ATGme

Plasmid

#163118

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

ffDronpa ATGme

Use

Synthetic Biology; Candida albicans expression

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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