We narrowed to 8,228 results for: pCMV

1. pCMV-PE2-SpRY-P2A-GFP

   Plasmid

   #159981

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpRY-P2A-GFP
   

   Use

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-SpG-P2A-EGFP (RTW4177)

   Plasmid

   #139988

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-PyronicSF/pCMV-myc-nuc

   Plasmid

   #124814

   Purpose

   Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

   Depositor

   Luis Felipe Barros

   Article

   Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

   Insert

   PyronicSF
   

   Use

   Tags

   Nuclear Localization Signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDF0234 pCMV - huDisCas7-11 dead mutant

   Plasmid

   #172509

   Purpose

   Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pCMV - huDisCas7-11 dead mutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Polymerase expression - pCMV-T7-EcKlenow (LM2692)

   Plasmid

   #208963

   Purpose

   Unfused EcKlenow DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   EcKlenow-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Neo-Bam p53 V143A

   Plasmid

   #16435

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 V143A (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V143A. Mutant cDNA from human colorectal tumor CX…

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-Neo-Bam p53 R249S

   Plasmid

   #16438

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R249S (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R249S

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCMV-intron myc Rab1a S25N

   Plasmid

   #46777

   Purpose

   expression of S25N Rab1a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   Rab1a S25N mutant (RAB1A Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S25N (dominant negative)

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

   Plasmid

   #200406

   Purpose

   Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   MCP-ADAR2dd(E488Q)-NES
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   E488Q (hyperactive)

   Promoter

   CMV

   Available since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE2-SpG-P2A-GFP

    Plasmid

    #159980

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

    Insert

    PE2-SpG-P2A-GFP
    

    Use

    Tags

    BpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-PEmax-P2A-EGFP (LM1589)

    Plasmid

    #223136

    Purpose

    pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized PEmax-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

    Expression

    Mammalian

    Mutation

    M-MLV mutations from PE2 (Anzalone et al. Nature …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-GABRA1(A322D)-IRES2-EGFP

    Plasmid

    #170821

    Purpose

    human GABAA receptors alpha1 subunit carrying A322D mutation and IRES2-EGFP

    Depositor

    Tingwei Mu

    Article

    Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

    Insert

    Human GABAA receptor alpha1 subunit carrying A322D mutation (GABRA1 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    change Alanine 322 to Aspartate

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-M13-TEV-C-P2A-TdTomato

    Plasmid

    #92389

    Purpose

    expresses Cal-light M13-TEV-C component and TdTomato in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    M13-TEV-C-P2A-TdTomato
    

    Use

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-AcrIIC3-NLS(SV40) (KAC209)

    Plasmid

    #134341

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC3 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC3
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV Fcgamma RIIa IRES neo

    Plasmid

    #14948

    Depositor

    Axel Nohturfft

    Article

    Trivedi et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

    Insert

    Fcgamma RIIa (FCGR2A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

    Plasmid

    #133801

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA4
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-ND1 Left -GSVG-UGI

    Plasmid

    #187412

    Purpose

    human mitochondrial DNA ND1 target TALE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA GSVG variant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

    Plasmid

    #231549

    Purpose

    Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    msfGFP (intramolecular)

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

    Plasmid

    #133802

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA5
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-PEmax-SpG-P2A-hMLH1dn

    Plasmid

    #211550

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG-P2A-hMLH1dn
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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