We narrowed to 13,645 results for: crispr cas9

1. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

   Plasmid

   #176836

   Purpose

   Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
   

   Use

   Unspecified

   Mutation

   See Depositor Comments Below

   Promoter

   Tet operator (tetracycline inducible expression)

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRISPRainbow Kit for Multiplex Labeling

   Plasmid Kit

   #1000000079

   Purpose

   CRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.

   Depositor

   Thoru Pederson

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #138149

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHEE-R-hyg9si

   Plasmid

   #222832

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, hygromycin and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHEE-R-hyg5A9si

   Plasmid

   #222835

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHEE-R-bar9si

   Plasmid

   #222834

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHEE-R-bar5A9si

   Plasmid

   #222837

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, bialaphos and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHEE-R-kan5A9si

   Plasmid

   #222836

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, kanamycin and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHEE-R-kan9si

   Plasmid

   #222833

   Purpose

   CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, kanamycin and red fluorescent seed selection)

   Depositor

   Hidenori Takeuchi

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. px330_SMAD exon 9 gRNA

    Plasmid

    #68352

    Purpose

    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Insert

    SMAD exon 9 gRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMZ379

    Plasmid

    #74215

    Purpose

    Combined sgRNA/Cas9 vector with NatMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMZ377

    Plasmid

    #74214

    Purpose

    Combined sgRNA/Cas9 vector with LEU2 marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST_140_LVL2 cam

    Plasmid

    #179334

    Purpose

    NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Mutation

    Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRISPRecise Kit

    Plasmid Kit

    #1000000235

    Purpose

    CRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.

    Depositor

    Ervin Welker

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

15. pGMC00010 (aka pRC0495)

    Plasmid

    #195295

    Purpose

    Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGMC00009 (aka pRC0494)

    Plasmid

    #195294

    Purpose

    Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMZ376

    Plasmid

    #74213

    Purpose

    Combined sgRNA/Cas9 vector with KanMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available Since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Promoter

    U6-96Ab and hsp70Bb

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDCC5

    Plasmid

    #59984

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    D10A

    Promoter

    hsp70Bb

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-module1

    Plasmid

    #222838

    Purpose

    Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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