Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,374 results for: pcDNA3.1+

Showing: 3881 - 3900 of 5374 results

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CARMEN

Plasmid

#240464

Purpose

Compartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ER

Depositor

Roland Malli

Article

Lischnig et al Br J Pharmacol. 2025 Oct 3. doi: 10.1111

Insert

CARMEN

Expression

Mammalian

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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IKKε v2-V5

Plasmid

#169770

Purpose

Express IKKε v2 in mammalian cells

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2

Tags

V5 tag

Expression

Bacterial and Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1369

Plasmid

#239828

Purpose

WT OrufIscB mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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Vinculin-Clover-CC-S1-mRuby2

Plasmid

#233237

Purpose

Vinculin FRET tension sensor with coiled-coil S1 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S1-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 JDM16-GFPnb-mCh

Plasmid

#235667

Purpose

Expresses designed inhibitor of Hsp70 tagged with a GFP nano body and mCherry

Depositor

David Baker

Article

Zhang et al Cell Chem Biol. 2025 Feb 4:S2451-9456(25

Insert

JDM16-GFPnb-mCh (Mch Synthetic)

Tags

GFP nanobody and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mZO-1(9-1665)-Myc-His

Plasmid

#236530

Purpose

For expression of ZO-1 (9-1665 aa, mouse)-Myc-His alpha- in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al Tissue Barriers. 2019;7(3):e1653748. doi

Insert

TJP1 (Tjp1 Mouse)

Tags

GFP and Myc-His

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mZO-1(9-1745)-Myc-His

Plasmid

#236529

Purpose

For expression of ZO-1 (9-1745 aa, mouse)-Myc-His alpha+ in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al Tissue Barriers. 2019;7(3):e1653748. doi

Insert

TJP1 (Tjp1 Mouse)

Tags

GFP and Myc-His

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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Vinculin-Clover-CC-S7-mRuby2

Plasmid

#233236

Purpose

Vinculin FRET tension sensor with coiled-coil S7 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S7-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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Vinculin-Clover-CC-S4-mRuby2

Plasmid

#233235

Purpose

Vinculin FRET tension sensor with coiled-coil S4 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S4-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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Vinculin-Clover-CC-S2-mRuby2

Plasmid

#233234

Purpose

Vinculin FRET tension sensor with coiled-coil S2 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S2-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

Plasmid

#231907

Purpose

For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-T2A-TagBFP

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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Const.4 EGFP-IRES-Luc-PS9

Plasmid

#231908

Purpose

For packaging EGFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S2-mRuby2-Vt

Plasmid

#233227

Purpose

Vinculin FRET tension sensor with coiled-coil S2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S2-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-M2-mRuby2-Vt

Plasmid

#233231

Purpose

Vinculin FRET tension sensor with coiled-coil M2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-M2-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vinculin-Clover-CC-Mad-mRuby2

Plasmid

#233239

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-Mad-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S1-mRuby2-Vt

Plasmid

#233230

Purpose

Vinculin FRET tension sensor with coiled-coil S1 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S1-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S0-mRuby2-Vt

Plasmid

#233226

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S0-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S4-mRuby2-Vt

Plasmid

#233228

Purpose

Vinculin FRET tension sensor with coiled-coil S4 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S4-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S7-mRuby2-Vt

Plasmid

#233229

Purpose

Vinculin FRET tension sensor with coiled-coil S7 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S7-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-Mad-mRuby2-Vt

Plasmid

#233232

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-Mad-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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