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pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pSMART-GFPuv

Plasmid

#65822

Purpose

GFPuv Expression, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Publication

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

gfpuv

Expression

Bacterial

Promoter

E . coli insulated waaH gene promoter, low phosp…

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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FLS-PC1

Plasmid

#41520

Depositor

Thomas Weimbs

Publication

Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Polycystin-1 (PKD1 Human)

Tags

Myc/His

Expression

Mammalian

Mutation

sequence from 4107 - 4303 of PKD1

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-ALPK2

Plasmid

#23493

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ALPK2 (ALPK2 Human)

Use

Gateway Cloning

Mutation

A117G

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MKNK1

Plasmid

#23531

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MKNK1 (MKNK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NME4

Plasmid

#23584

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME4 (NME4 Human)

Use

Gateway Cloning

Mutation

1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Stx5L-mCherry

Plasmid

#209844

Purpose

Expresses Stx5L-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Publication

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

STX5 (STX5 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Wildtype Stx5 (with 55M)

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA1_ASCL1

Plasmid

#64129

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL1 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-BCKDK

Plasmid

#23794

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BCKDK (BCKDK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
RUNX1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176974

Purpose

Mammalian expression vector encoding RUNX1 and EmGFP-Bsd

Depositor

Roger Reeves

Publication

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

RUNX1 (RUNX1 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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BII-BCA-Prom-ASCL2

Plasmid

#133394

Purpose

Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

Depositor

Blair Madison

Publication

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

destabilized tdTomato-NLS and destabilized NLS-GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free) drives expressi…

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMesh1*

Plasmid

#175591

Purpose

Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

Depositor

Greg Bokinsky

Publication

Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

Insert

Mesh1 (Mesh1 Fly)

Tags

Fused with a flexible GS linker to cerulean

Expression

Bacterial

Mutation

Codon optimized for translation in E.coli. AGGAGG…

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GR-AKAR3

Plasmid

#173015

Purpose

Improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Publication

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

GR-AKAR3

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 WT in pcDNA3.1

Plasmid

#118213

Purpose

Expresses HA-UBE2E1 in mammalian cells

Depositor

Cynthia Wolberger

Publication

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

none

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-GFP-SFPQ

Plasmid

#97084

Purpose

Encodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.

Depositor

Archa Fox

Publication

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA GFP-SFPQ

Use

CRISPR

Promoter

U6

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-KSR2

Plasmid

#23501

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KSR2 (KSR2 Human)

Use

Gateway Cloning

Mutation

aa344-5 (DP) deleted; S523G compared to GenBank E…

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-TTK

Plasmid

#23857

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TTK (TTK Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCORE-Kp53

Plasmid

#72241

Purpose

Contains the CORE cassette with KanMX4 and human p53 gene markers

Depositor

Michael Resnick, Francesca Storici

Publication

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

p53 (TP53 Human)
kanMX4

Expression

Bacterial

Mutation

V122A mutation

Available since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet1 promoter A

Plasmid

#63881

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Publication

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment A

Use

Luciferase

Expression

Mammalian

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-4T1-WDR5

Plasmid

#59969

Purpose

Bacterial Expression GST-WDR5

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Tags

GST

Expression

Bacterial

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles