We narrowed to 6,366 results for: wus

1. PX459_GRHL1-exon5

   Plasmid

   #177763

   Purpose

   sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX459_GRHL1-exon8

   Plasmid

   #177764

   Purpose

   sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL1 (GRHL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdestMB14_grh-1p-grh-1-gfp

   Plasmid

   #177761

   Purpose

   Overexpression of C. elegans grh-1

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   grh-1 (grh-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pdestMB14_hsp-16.2p-grh-1-gfp

   Plasmid

   #177762

   Purpose

   Overexpression of C. elegans grh-1

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   grh-1 (grh-1 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRB131

   Plasmid

   #180174

   Purpose

   pAAV construct GluN1A714L (shRNA resistant)

   Depositor

   Richard Bergeron

   Article

   Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

   Insert

   GluN1 (Grin1 Rat)
   

   Use

   AAV

   Tags

   Phluorin

   Expression

   Mammalian

   Mutation

   A714L

   Promoter

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR207-MUM2sp-Citrine-ADPG2

   Plasmid

   #170725

   Purpose

   Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

   Depositor

   George Haughn

   Article

   McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

   Insert

   ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
   

   Use

   Gateway donor vector / entry clone

   Tags

   Citrine tag (714bp)

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LV_EF1a_MAPK9-P2A-Hygro_Barcode

   Plasmid

   #170238

   Purpose

   Barcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAPK9 (MAPK9 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDestTol2-actinb-kcnj13-M135R-EGFP

   Plasmid

   #164944

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135R

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnj13 (kcnj13 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   EGFP

   Expression

   Mutation

   Stop codon deleted; M135R

   Promoter

   actinb (actb2) promoter

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pInducer20 CARD8-HA PAM L368G

   Plasmid

   #169985

   Purpose

   Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutations

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   PAM, L368G

   Promoter

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pInducer20 CARD8-HA PAM R394E

    Plasmid

    #169987

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, R394E

    Promoter

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pInducer20 CARD8-HA PAM F405G

    Plasmid

    #169988

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, F405G

    Promoter

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pInducer20 CARD8-HA PAM F370G

    Plasmid

    #169986

    Purpose

    Tet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    PAM, F370G

    Promoter

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221 Ub-NLRP1 L1215

    Plasmid

    #166828

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221 Ub-NLRP1 S1213

    Plasmid

    #166827

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR221 NLRP1 T755N

    Plasmid

    #166820

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    T755N

    Promoter

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 NLRP1 R726W

    Plasmid

    #166819

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    R726W

    Promoter

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221 Ub-NLRP1 L1218

    Plasmid

    #166829

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

    Plasmid

    #164945

    Purpose

    Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    KCNJ13 (KCNJ13 Human)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Stop codon deleted

    Promoter

    actinb (actb2) promoter

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDestTol2-actinb-kcnj13-Q153H-EGFP

    Plasmid

    #164943

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted; Q153H; M154I

    Promoter

    actinb (actb2) promoter

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDestTol2-actinb-kcnj13-T131A-EGFP

    Plasmid

    #164942

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted; T131A; G133R

    Promoter

    actinb (actb2) promoter

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge