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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,023 results for: golden gate

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Showing: 3901 - 3920 of 5023 results

  1. pEPOR1CB0002

    Plasmid
    #117543
    Purpose
    Level 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plants
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421)
    Expression
    Plant
    Promoter
    2x35S+5'UTR OMEGA (pICH51288)
    Available Since
    Nov. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    117543-plasmid-map-sequence-id-228068

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  2. Level 1 P1 OsActinP:Hygint:NosT

    Plasmid
    #165423
    Purpose
    GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    Use
    Synthetic Biology; L1 p1(r) golden gate (moclo) h…
    Promoter
    Os Actin promoter :: Hygromycin resistance gene (…
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165423-plasmid-map-sequence-id-331267

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  9. pBLU-VIPR

    Plasmid
    #220498
    Purpose
    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al Nucleic Acids Res. 2025 Mar 20;53(6):gka
    Type
    Empty backbone
    Use
    CRISPR; Transposon
    Expression
    Mammalian
    Available Since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220498-plasmid-map-sequence-id-442816

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  10. pTU2-A-RFP (p15A origin)

    Plasmid
    #74090
    Purpose
    pTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    R0010 Const. Promoter controlling RFP
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74090-plasmid-map-sequence-id-138311

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  11. pTU2S-b (p15A origin)

    Plasmid
    #74089
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74089-plasmid-map-sequence-id-138299

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  14. pTL50_cHS4-CAG-s36GFPmNG-t2A-PuroR-p2A-BFP-polyAcHS4

    Plasmid
    #223541
    Purpose
    PUFFFIN cassette with s36GFP-mNeonGreen and tagBFP, strong constitutive promoter CAG, insulated by cHS4 for random integration, puromycin resistance for selection in mammalian cells
    Depositor
    Sally Lowell
    Article
    Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318
    Insert
    s36GFP-mNeonGreen, tagBFP, PAC
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    223541-plasmid-map-sequence-id-442162

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Showing: 3901 - 3920 of 5023 results