We narrowed to 6,456 results for: golden gate
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Plasmid#228639PurposeBacterial expression of the Coxsackievirus 33 isoform B 3C proteaseDepositorInsertCoxsackievirus 33 isoform B 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHW1385
Plasmid#229879PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTRDepositorInsertPmyo-2::HisCl1 cDNA::SL2::mScarlet-I-C1::tbb-2 3'UTR
TagsmScarlet-I-C1ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
MbCas12a_K487_GPG_GR2
Plasmid#231577PurposeEncodes a cortisol inducible variant of MbCas12aDepositorInsertMbCas12a with a GR2 insertion behind K487
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
PDCD1-DEM (P1#5)
Plasmid#227738PurposeDesigner Epigenome Modifier (DEM) designed to target the PDCD1-promoterDepositorInsertDesigner epigenome modifier targeting PDCD1-promoter
UseTALENTagsDNMT3A, DNMT3L, KRABExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LAG3-DEM (L3#1)
Plasmid#227739PurposeDesigner Epigenome Modifier (DEM) designed to target the LAG3-promoterDepositorInsertDesigner epigenome modifier targeting LAG3-promoter (Homo sapiens)
UseTALENTagsDNMT3A, DNMT3L, KRABExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330_SYCP3_nterm
Plasmid#222524PurposeCas9/sgRNA plasmid for targeting SYCP3DepositorInsertCas9, SYCP3 sgRNA (SYCP3 Synthetic, Human)
UseCRISPRAvailable SinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML343_RAB14-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227908PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertRAB14 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML325_GPR107-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227909PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertGPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227916PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227891PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertVPS35 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227894PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC61B homology arms with mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227896PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCCDC47 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227900PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227901PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertMAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227905PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCOPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKSEe401R
Plasmid#218532PurposeCRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.DepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC
Plasmid#229545PurposeLentiviral construct to express rtTA and SKM proteinsDepositorInsertrTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
UseLentiviralExpressionMammalianPromoterhEF1aAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTBL3328 PB-sfGFP-WPRE-target
Plasmid#226676PurposePiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.DepositorInsertsfGFP-WPRE-site3target
ExpressionMammalianPromoterEF1alphaAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVP183
Plasmid#222050PurposeGreenGate DF bridge module containing filler sequence.DepositorInsertFiller
UseSynthetic Biology; Greengate compatible cloning v…Available SinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP077
Plasmid#222029PurposeEmpty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…MutationAll Esp3i sites domesticatedAvailable SinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits