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pNM3725

Plasmid

#154113

Purpose

UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

UAS 11X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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CKAR-T/A

Plasmid

#14861

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Mutation

T/A

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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CKAR-T/A

Plasmid

#14861

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Mutation

T/A

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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PAS528 (TJF059)

Plasmid

#65602

Purpose

pETDuet-1 Containing FadD-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Promoter

T7 lacO

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
PAS528 (TJF059)

Plasmid

#65602

Purpose

pETDuet-1 Containing FadD-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Promoter

T7 lacO

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pJF093

Plasmid

#113323

Purpose

Sp.pCas9-dCas9, pTet-MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJF093

Plasmid

#113323

Purpose

Sp.pCas9-dCas9, pTet-MCP-SoxS_R93A

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9 and MCP-SoxS_R93A

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-DHFR-myc

Plasmid

#118852

Purpose

For fluorophore translocation assay and inducible tagging with zapalog.

Depositor

Thomas Schwarz

Article

Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

Type

Empty backbone

Tags

DHFR, Myc, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-DHFR-myc

Plasmid

#118852

Purpose

For fluorophore translocation assay and inducible tagging with zapalog.

Depositor

Thomas Schwarz

Article

Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

Type

Empty backbone

Tags

DHFR, Myc, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBA2675

Plasmid

#189997

Purpose

Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)

Depositor

Bungo Akiyoshi

Article

Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

Inserts

FBP75
VhhGFP4

Use

Expression vector for trypanosoma brucei

Tags

NLS

Mutation

N-terminal 600 bp of FBP75

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBA2675

Plasmid

#189997

Purpose

Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)

Depositor

Bungo Akiyoshi

Article

Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do

Inserts

FBP75
VhhGFP4

Use

Expression vector for trypanosoma brucei

Tags

NLS

Mutation

N-terminal 600 bp of FBP75

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2J2

Plasmid

#15794

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2J2

Plasmid

#15794

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pSP561

Plasmid

#139497

Purpose

Expression vector for the MS2-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 MS2-GFPenvy tCYC1

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSP561

Plasmid

#139497

Purpose

Expression vector for the MS2-GFP protein in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pADH1 MS2-GFPenvy tCYC1

Expression

Yeast

Mutation

∆FG

Promoter

pADH1

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-miR30

Plasmid

#20670

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Insert

miR-30 (MIR30A Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-miR30

Plasmid

#20670

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Insert

miR-30 (MIR30A Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Expression

Worm

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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