We narrowed to 10,779 results for: T7

Showing: 3921 - 3940 of 10779 results

Results per page:

Sort results by:

pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-SUPREM

Plasmid

#205023

Purpose

Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

SUPer Rna Ecogii Methyltransferase

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-AncMEcoGII291

Plasmid

#205024

Purpose

Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 291

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a-hPKM2

Plasmid

#44242

Depositor

Lewis Cantley, Matthew Vander Heiden

Article

Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

Insert

Pyruvate Kinase M2 (PKM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Article

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM4Z-EGFP

Plasmid

#183475

Purpose

This plasmid can be used to prepare EGFP mRNA by In Vitro Transcription

Depositor

Chantal Pichon

Article

Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

Insert

EGFP

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pALOD4

Plasmid

#111026

Purpose

Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1C3-pT7-sfGFP_RED20

Plasmid

#135173

Purpose

Expresses sfGFP recoded in the RED20 genetic code off the T7 promoter

Depositor

Drew Endy

Article

Calles et al Nucleic Acids Res. 2019 Sep 12. pii: 556

Insert

sfGFP_RED20

Use

Synthetic Biology

Mutation

Recoded ORF of sfGFP to only use one codon per am…

Promoter

taatacgactcactata

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
V48 ELP

Plasmid

#68395

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V48 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
6BHD (SETDB1)

Plasmid

#119955

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

SETDB1 (SETDB1 Human)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

contains amino acids 190-410

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET30b-7+-SrtA

Plasmid

#105602

Purpose

expressed 7+ sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7+ mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-CalfluxVTN

Plasmid

#83926

Purpose

Bioluminescent Reporter of Intracellular Calcium

Depositor

Carl Johnson

Article

Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

Insert

CalfluxVTN

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS1_Opt_Renilla

Plasmid

#210571

Purpose

Expresses Optimal Renilla luciferase

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Optimal Renilla

Use

Luciferase

Expression

Mammalian

Promoter

HSV TK; T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21b Hot-PETase

Plasmid

#225761

Purpose

Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

Hot-PETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11b-SpyCatcher-HA2

Plasmid

#165991

Purpose

SpyCatcher-fused influenza HA ministem with removable HisTag

Depositor

Vesa Hytönen

Article

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

SpyCatcher-HA2

Tags

Foldon, HisTag, and SpyCatcher

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SPYtag-Histag-Avidin

Plasmid

#214836

Purpose

This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

SPYtag-Histag-Avidin

Expression

Bacterial

Promoter

T7

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/HisB (TIREVOL)-mEos3.2

Plasmid

#189720

Purpose

titratable expression of mEos3.2 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mEos3.2

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge