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lvl0 lexA-35S

Plasmid

#153407

Purpose

Estradiol system inducible promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

lexA-35S promoter

Expression

Plant

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGGA000

Plasmid

#48856

Purpose

Empty entry vector for creating GreenGate promoter modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P*-R

Plasmid

#189850

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P*-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P* (modified T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCasX1

Plasmid

#87685

Purpose

p15A plasmid expressing CasX locus from Deltaproteobacteria

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasX1

Expression

Bacterial

Promoter

None

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pZa-tetO-csgB-CmR

Plasmid

#89580

Purpose

Express csgB curli protein

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgB

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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p15aC-1D

Plasmid

#107863

Purpose

Bacterial expression of PIS

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

PIS

Tags

myc

Expression

Bacterial

Promoter

proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGGC000

Plasmid

#48858

Purpose

Empty entry vector for creating GreenGate coding sequence modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pVTU-Dest

Plasmid

#105088

Purpose

Yeast expression vector for Gateway cloning and SUS bridge assays

Depositor

Christopher Grefen

Article

Grefen et al Methods Mol Biol. 2009;479:217-33. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-knt-P**-R

Plasmid

#189851

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P**-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P** (modified T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-cat-P-R

Plasmid

#189853

Purpose

Cre-inducible activation of cat for chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-cat

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pNJB80

Plasmid

#51518

Purpose

Gateway entry vector with attL5 and attL2 sites flanking a CmR and ccdB gene. In our hands, the multisite reaction still proceeds efficiently.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Type

Empty backbone

Use

Gateway entry vector

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE 1397C

Plasmid

#221936

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE 1397N

Plasmid

#221937

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

TALE: Q231R, W232G, S233A

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX DdCBE DddA6-11 1397C

Plasmid

#221933

Purpose

Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397C

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Mutation

DddA: T1413I

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBbS5c-Maqu_3494

Plasmid

#86205

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Expression

Bacterial

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP

Plasmid

#134357

Purpose

Reporter plasmid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Inserts

GFP
mCherry

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGCS-C3m(3xFLAG)

Plasmid

#85726

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xFLAG

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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p7XNH3

Plasmid

#47064

Purpose

FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-131

Plasmid

#185923

Purpose

Himar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mTurquoise2

Expression

Bacterial

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGGF000

Plasmid

#48861

Purpose

Empty entry vector for creating GreenGate plant resistance cassette modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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