We narrowed to 266,092 results for: des

1. MSCV-CMV-DsRed-IRES-EGFP-DEST

   Plasmid

   #41941

   Depositor

   Stephen Elledge

   Article

   Yen et al Science. 2008 Nov 7;322(5903):918-23. do

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST40-2XFL-Src-K298M-Y419F-Y530F

   Plasmid

   #140321

   Purpose

   Expresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   K298M-Y419F-Y530F

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)

   Plasmid

   #132926

   Purpose

   pMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Insert

   Luc2P
   

   Use

   Luciferase and Synthetic Biology; Pmvp gateway en…

   Tags

   PEST destabilization domain

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-LyzC-RacFRET-U6ac-ctrl guides

   Plasmid

   #168247

   Purpose

   "label active Rac; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   RacFRET
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

   Plasmid

   #42651

   Purpose

   destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 399 SNP (TLR4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T399I

   Promoter

   CMV/TO

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti CMV Puro DEST (w118-1) SDC3

   Plasmid

   #107501

   Purpose

   Expresses SDC3, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   SDC3 (codon optimized) (SDC3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized sequence

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Affibody HER2:243.BirA*-pET301/NT-DEST

   Plasmid

   #174692

   Purpose

   Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*

   Depositor

   Jean Gariepy

   Article

   Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

   Insert

   Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   R118G mutation in BirA

   Promoter

   T7 promoter

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

   Plasmid

   #40132

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease (ELD)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

   Plasmid

   #40131

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Use

   Tags

   FokI nuclease (KKR)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF5/FRT/DEST-3xHA/mGli3/Flag

    Plasmid

    #51246

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLenti CMV Puro DEST (w118-1) eGFP

    Plasmid

    #107505

    Purpose

    Expresses eGFP, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

    Plasmid

    #52505

    Purpose

    mammalian expression vector of 53BP1 28A mutant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    53BP1 28A (TP53BP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    All 28 SQ/TQ motifs in N-term of 53BP1 have been …

    Promoter

    Available since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLenti CMV Puro DEST (w118-1) PHLDB1

    Plasmid

    #107503

    Purpose

    Expresses PHLDB1, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    PHLDB1 (PHLDB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Nucleotide changes 1236 T>C, 1365 C>T, 1371…

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV Puro DEST (w118-1) EPB41L1

    Plasmid

    #107508

    Purpose

    Expressing EPB41L1, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    EPB41L1 (EPB41L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3_attR1-ORF-attR2-NTEV-TCS-GV-2xHA_DEST

    Plasmid

    #194385

    Purpose

    Gateway Destination vector for split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    attR1-ORF-attR2-NTEV-TCS-GV-2xHA
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV Puro DEST (w118-1) IGF1R

    Plasmid

    #107499

    Purpose

    Expresses IGF1R, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    IGF-1R (IGF1R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

    Plasmid

    #42650

    Purpose

    destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV/TO

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. mmu-miR-30a-5p Block-iT design (BLK)

    Plasmid

    #107789

    Purpose

    miR-30a RNAi Expression

    Depositor

    Fabio Penna

    Insert

    Mmu-miR-30a-5p mature sequence (Mir30a Mouse)
    

    Use

    RNAi

    Tags

    EmGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKB11 (pDEST-DHFR F[1,2] C-term, NatR)

    Plasmid

    #210485

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)

    Plasmid

    #210487

    Purpose

    pDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector for gateway…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge