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Plasmid#121997PurposeExpression of MFN2 and mitoRFP in mammalial cellsDepositorInsertMFN2-IRES-mitoRFP (MFN2 Human)
UseRetroviralTagsExpressionMutationsilent Q386Q Q387Q V388V Y389Y C390C E391E E392EPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Hygro-Mfn2-ActA
Plasmid#191981PurposeExpression of Mfn2 tagged with mitochondrion localization signal (ActA)DepositorInsertMfn2-ActA
UseTagsActAExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcB6-Myc-Mfn2 IYFFT
Plasmid#191953PurposeExpression of Mfn2 protein, in which the C-terminus, after the transmembrane domain, is replaced by a stretch of hydrophobic amino acids (IYFFT)DepositorInsertMyc-Mfn2-IYFFT
UseTagsExpressionMammalianMutationC- terminus replaced by the stretch of hydrophobi…PromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-MFN2-Strep (SB253)
Plasmid#227602PurposeInducible expression of Strep-tagged MFN2 in Pichia pastorisDepositorInsertMFN2 (MFN2 Human)
UseTagsTwin-StrepTag, 10xHisExpressionYeastMutationPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mito-GFP-ER tethering
Plasmid#160509PurposeRescue mfn2 knockdown by tethering mitochondria and ER with GFPDepositorInsertmito-GFP-ER tethering
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-mfn2 guide
Plasmid#121993PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-mfn2 guide#2
Plasmid#121994PurposeAn entry vector with U6a and U6c promoter driving mfn2 guide RNAs expressionDepositorInsertmfn2 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)
Plasmid#227608PurposeInducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)
Plasmid#227609PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-544, 651-757 only [TM deleted]PromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2-Strep (SB255)
Plasmid#227604PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationPromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only